ATCC? Number:30884?
Organism: Acanthamoeba royreba Willaert et al.
Designations: Oak Ridge [AC-023]
Isolation: BeWo human choriocarcinoma cells, Oak Ridge, TN, 1975
Depositors: E Willaert
History: ATCC<<--E Willaert<<--R.L. Tyndall
Biosafety Level:1
Shipped: freeze-dried
Growth Conditions: ATCCmedium 712: PYGTemperature: 25.0°C Duration: axenic Protocol: ATCCNO: 30010 SPEC: This strain is distributed as a freeze-dried preparation. See the general procedures for opening a freeze-dried vial. Aseptically add 0.5 ml of ice cold medium containing 12% (w/v) sucrose to the freeze-dried inner shell vial. Once the culture is completely rehydrated, aseptically add 1 ml of ATCCmedium 712 and distribute to a 16 X 125 mm plastic screw-capped test tube or a T-25 tissue culture flask containing 5.0 ml of the same medium. Incubate the test tube culture horizontally with the cap on tight. Trophozoites should be evident in 1-5 days.
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Type Strain: yes(type strain)
Comments: Taxonomy based on isoenzyme profiles and rDNA PCR-RFLP patterns [32205] species description [4611] [4664] [4831] characterization of Acanthamoeba polyphaga [4673] review [23576] phylogeny [24028]
Cross References: Nucleotide (GenBank) : U07417 nuclear SSU rRNA gene
Classification: KINGDOM: Protozoa