ATCC? Number:30876?
Organism: Naegleria gruberi Schardinger
Designations: CCAP-1518/e
Isolation: freshwater, Drummond, WI, 1964
Depositors: CCAP
History: ATCC<<--CCAP<<--F.C. Page, strain 24
Biosafety Level:1
Shipped: frozen
Growth Conditions: ATCCmedium 997: Fresh water ameba mediumTemperature: 25.0°C Duration: grown with Escherichia coli ATCC11775 Protocol: ATCCNO: 30133 SPEC: This strain is shipped as a dried preparation. Aseptically add 1 ml of sterile distilled water to the inner vial, remove the filter paper pellet with a pair of forceps, and place it in the center of an agar plate of ATCCmedium 711 or 997. Add the liquid remaining in the vial to the plate and spread it evenly over the surface of the plate. Incubate the plate at 25C. Trophozoites (amebae) should be evident within 2-3 days.
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Comments: Zymodeme Gr-4 [23932] Maximum temperature tolerance 37C [23932] This strain is a member of Naegleria sp. genetic cluster E Interrepeat PCR [23881] biochemical identification [4799]
References: 4799: Daggett PM, Nerad TA. The biochemical identification of vahlkampfiid amoebae. J. Protozool. 30: 126-128, 1983. PubMed: 6864593 4936: Page FC. Morphological variation in the cyst wall of Naegleria gruberi (amoebida, vahlkampfiidae). Protistologica 11: 195-204, 1975. 23881: van Belkum A, et al. Genotyping Naegleria spp. and Naegleria Fowleri isolates by Interepeat Polymerase Chain reaction. J. Clin. Microbiol. 30: 2595-2598, 1992. PubMed: 1400959 23932: Adams M, et al. A genetic approach to species criteria in the amoeba genus Naegleria using allozyme electrophoresis. Int. J. Parasitol. 19: 823-834, 1989. PubMed: 2635158 61465: De Jonckheere JF. A century of research on the amoeboflagellate genus Naegleria. Acta Protozool. 41: 309-342, 2002.