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ATCC® Number:30873™
Organism: Acanthamoeba polyphaga (Puschkarew) Page
Designations: CCAP 1501/3d
Isolation: human corneal ulcer, Cambridge, England, 1974
Depositors: CCAP
History: ATCC<<--CCAP<<--J. Nagington
Biosafety Level:2
Shipped: frozen
Growth Conditions: ATCCmedium 711: PYBATCCmedium 997: Fresh water ameba mediumTemperature: 25.0℃ Growth condition: xenic with Enterobacter aerogenes ATCC13048
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Comments: Genetic markers that distinguish pathogenic and nonpathogenic strains [31825] case report [4588] Contact lens disinfection [21716] Effect of contact lens on disinfection [4564] phylogeny [24028] ATCCmedium 711 is richer than ATCCmedium 997 and may produce denser and faster bacterial growth. Excess bacterial growth may inhibit growth of amoebae.
Cross References: Nucleotide (GenBank) : AF019062 Acanthamoeba polyphaga Nagington 18S ribosomal RNA gene, partial sequence.
Classification: KINGDOM: Protozoa
References: 4564: Silvany RE, et al. The effect of currently available contact lens disinfection systems on Acanthamoeba castellanii and Acanthamoeba polyphaga. Ophthalmology 97: 286-290, 1990. PubMed: 2336265 4588: Naginton J, et al. Amoebic infection of the eye. Lancet 2: 1537-1540, 1974. PubMed: 4140981 21716: Silvany RE, et al. Effect of contact lens preservatives on Acanthamoeba. Ophthalmology 98: 854-857, 1991. PubMed: 1866136 23896: Daggett PM, et al. Distribution and possible interrelationships of pathogenic and nonpathogenic Acanthamoeba from aquatic environments. Microb. Ecol. 8: 371-386, 1982. 24028: Daggett PM, et al. A molecular approach to the phylogeny of Acanthamoeba. Biosystems 18: 399-405, 1985. PubMed: 4084681 31825: Howe D, et al. Identification of two genetic markers that distinguish pathogenic and nonpathogenic strains of Acanthamoeba spp.. Parasitol. Res. 83: 435-348, 1997. PubMed: 9197389 32162: Stothard DR, et al. The evolutionary history of the genus Acanthamoeba and the identification of eight new 18S rRNA gene sequence types. J. Eukaryot. Microbiol. 45: 45-54, 1998. PubMed: 9495032 32325: Alizadeh H, et al. In vitro amoebicidal activity of propamidine and pentamidine isethionate against Acanthamoeba species and toxicity to corneal tissues. Cornea 16: 94-100, 1997. PubMed: 8985640 33455: Kong HH, et al. Interstrain polymorphisms of isoenzyme profiles and mitochondrial DNA fingerprints among seven strains assigned to Acanthamoeba polyphaga. Korean J. Parasitol. 33: 331-340, 1995. PubMed: 8591011 34596: Niszl IA, et al. Cytopathogenicity of clinical and environmental Acanthamoeba isolates for two mammalian cell lines. J. Parasitol. 84: 961-967, 1998. PubMed: 9794638 57068: Kong HH, et al. Mitochondrial DNA restriction fragment length polymorphism (RFLP) and 18S small-subunit ribosomal DNA PCR-RFLP analyses of Acanthamoeba isolated from contact lens storage cases of residents in southwestern Korea. J. Clin. Microbiol. 40: 1199-1206, 2002. PubMed: 11923331 70201: Alves JMP, et al. Random amplified polymorphic DNA probes as a tool for the characterization of Brazilian keratitis isolates of the genus Acanthamoeba. Braz. J. Med. Biol. Res. 33: 19-26, 2000. 70805: Ledee DR, et al. Advantages of using mitochondrial 16S rDNA sequences to classify clinical isolates of Acanthamoeba. Invest. Ophthalmol. Vis. Sci. 44: 1142-1149, 2003. PubMed: 12601042 71070: Yagita K, et al. Clustering of Acanthamoeba isolates from human eye infections by means of mitochondrial DNA digestion patterns. Parasitol. Res. 85: 284-289, 1999. PubMed: 10099009