ATCC? Number:30896?
Organism: Naegleria fowleri Carter
Designations: 6088
Isolation: human cerebrospinal fluid of 9-year-old female, Deep Creek Hot Springs, San Bernardino National Forest, CA, 1978
Depositors: FL Schuster
History: ATCC<<--FL Schuster<<--T.H. Dunnebacke <<--- C. Powers <<--- J. Seidel
Biosafety Level:2
Shipped: frozen
Growth Conditions: ATCCmedium 1034: Modified PYNFH medium (Available from ATCCas ATCCcat. no. 327-X)Temperature: 35.0°C Duration: axenic
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Comments: Case successfully treated with drugs. Restriction endonuclease analysis of mitochondrial DNA [4813] biochemical identification [4799] differentiation of Naegleria fowleri from Acanthamoeba using monocolonal antibody [24171]
References: 4799: Daggett PM, Nerad TA. The biochemical identification of vahlkampfiid amoebae. J. Protozool. 30: 126-128, 1983. PubMed: 6864593 4813: Milligan SM, Band RN. Restriction endonuclease analysis of mitochondrial DNA as an aid in the taxonomy of Naegleria and Vahlkampfia. J. Protozool. 35: 198-204, 1988. PubMed: 2840491 5095: Powers JS, Abbot R. Epidemiologic notes and reports: Primary amebic meningoencephalitis - California, Florida, New York. Morb. Mortal. Wkly. Rep. 27: 343-344, 1978. 24171: Flores BM, et al. Differentiation of Naegleria fowleri from Acanthamoeba species by using monoclonal antibodies and flow cytometry. J. Clin. Microbiol. 28: 1999-2005, 1990. PubMed: 2229384