ATCC? Number:30882?
Organism: Leishmania major (Yakimoff and Schokhor) Bray et al.
Designations: Jericho II [WR 061c]
Isolation: reisolated from patient with a vaccinated inoculation of LRC-L136 (Jericho), Israel, Occupied Territory, 1967
Depositors: WRAIR
History: ATCC<<--WRAIR<<--B. Walton <<--- Z. Greenberg strain LRC-L137
Biosafety Level:2
Shipped: frozen
Growth Conditions: ATCCmedium 807: Brain heart infusion blood agarTemperature: 25.0°C Duration: axenic Protocol: ATCCNO: 11745 SPEC: See general instructions for thawing and storage of frozen material before proceeding. Add thawed contents to a single 16 x 125 mm glass screw-capped test tube of the appropriate medium. Incubate the culture vertically with the cap screwed on tightly. It is essential to establish cultures initially in small volumes. Once established, the culture can be scaled up to larger volumes. Vigorously agitate the culture and aseptically transfer 0.1 ml of culture to a fresh tube of medium weekly.
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Applications: used to vaccinate against L. tropica [57555]
Comments: Promastigotes.
References: 57555: Naggan L, et al. Follow-up study of a vaccination programme against cutaneous leishmaniasis. II. Vaccination with a recently isolated strain of L. tropica from Jericho. Trans. R. Soc. Trop. Med. Hyg. 66: 239-243, 1972. PubMed: 5048790