ATCC® Number:30888™
Organism: Giardia intestinalis (Lambl) Alexeieff
Designations: Portland-1
Isolation: human female, Portland, OR, 1971
Depositors: LS Diamond
History: ATCC<<--LS Diamond<<--T.S. Visvesvara <<--- E.A. Meyer
Biosafety Level:2
Shipped: frozen
Growth Conditions: ATCCmedium 2155: LYI Giardia Medium (filtered)Temperature: 35.0℃ Duration: axenic; anaerobic
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Comments: Isoenzyme electrophoresis [5021] not detected by Trichomonas vaginalis-specific PCR primers [37722] Respiratory metabolism [4977] Equivalency of two nuclei [4839] Factors influencing attachment of flagellate [21702] Excretory/secretory products [21704] Binding of antidepressants to trophozoites [21714] Molecular comparison of strains based upon RFLPs of glutamate dehydrogenase gene [21763] Restriction - endonuclease analysis of DNA [4720] Characterization of calmodulin [23864] Critical comparison using isoenzyme electrophoresis [23916] killing by human milk [23967] Characterization of isolates from a waterbrone outbreak [5021] Inhibition of uridine phosphorylase by pyrimidine analogs [24000] Mass cultivation [24013] Comparison of isoenzymes [24034] simple method of cloning [24093] Inhibition of growth by difluoromethyl-ornithine [24153] Ultrastructure localization of giardins [24213]
Related Items: purified DNA:ATCC30888D
Classification: KINGDOM: Archeozoa
References: 4720: Nash TE, et al. Restriction-endonuclease analysis of DNA from 15 Giardia isolates obtained from humans and animals. J. Infect. Dis. 152: 64-73, 1985. PubMed: 2409186 4839: Kabnick KS, Peattie DA. In situ analyses reveal that the two nuclei of Giardia lamblia are equivalent. J. Cell Sci. 95: 353-360, 1990. PubMed: 2384520 4977: Weinbach EC, et al. Respiratory metabolism of Giardia lamblia. J. Parasitol. 66: 347-350, 1980. PubMed: 6248623 5021: Isaac-Renton JL, et al. Characterization of Giardia duodenalis isolates from a waterborne outbreak. J. Infect. Dis. 167: 431-440, 1993. PubMed: 8421176 21702: Gillin FD, Reiner DS. Attachment of the flagellate Giardia lamblia: Role of reducing agents, serum, temperature, and ionic composition. Mol. Cell. Biol. 2: 369-377, 1982. PubMed: 7110136 21704: Nash TE, et al. Excretory-secretory products of Giardia lamblia. J. Immunol. 131: 2004-2010, 1983. PubMed: 6619547 21714: Weinbach EC, et al. Binding of tricyclic antidepressant drugs to trophozoites of Giardia lamblia. Comp. Biochem. Physiol. 102: 391-396, 1992. PubMed: 1360349 21763: Monis PT, et al. Molecular genetic analysis of Giardia intestinalis isolates at the glutamate dehydrogenase locus. Parasitology 112: 1-12, 1996. PubMed: 8587793 23864: Munoz ML, et al. Giardia lamblia: Detection and characterization of calmodulin. Exp. Parasitol. 63: 42-48, 1987. PubMed: 3026834 23905: McCabe RE, et al. In vitro model of attachment of Giardia intestinalis trophozoites to EIC-6 cells, an intestinal cell line. Antimicrob. Agents Chemother. 35: 29-35, 1991. PubMed: 1901700 23916: Meloni BP, et al. Critical comparison of Giardia duodenalis from Australia and Switzerland using isoenzyme electrophoresis. Acta Trop. 50: 115-124, 1992. PubMed: 1685867 23967: Gillin FD, et al. Human milk kills parasitic intestinal protozoa. Science 221: 1290-1292, 1983. PubMed: 6310751 24000: Jimenez BM, et al. Inhibition of uridine phosphorylase from Giardia lamblia by pyrimidine analogs. Biochem. Pharmacol. 38: 3785-3789, 1989. PubMed: 2597172 24013: Wieder SC, et al. Mass cultivation of Giardia lamblia in a serum-free medium. J. Parasitol. 69: 1181-1182, 1983. PubMed: 6674471 24031: Gillin FD, Diamond LS. Clonal growth of Giardia lamblia trophozoites in a semisolid agarose medium. J. Parasitol. 66: 350-352, 1980. PubMed: 7391877 24034: Bertram MA, et al. A comparison of isozymes of five axenic Giardia isolates. J. Parasitol. 69: 793-801, 1983. PubMed: 6672161 24093: Binz N, et al. A simple method for cloning Giardia duodenalis from cultures and fecal samples. J. Parasitol. 77: 627-631, 1991. PubMed: 1865272 24153: Gillin FD, et al. Inhibition of growth of Giardia lamblia by difluoromethylornithine, a specific inhibitor of polyamine biosynthesis. J. Protozool. 31: 161-163, 1984. PubMed: 6330350 24213: Peattie DA, et al. Ultrastructural localization of giardins to the edges of disk microribbons of Giarida lamblia and the nucleotide and deduced protein sequence of alpha giardin. J. Cell Biol. 109: 2323-2335, 1989. PubMed: 2808530 32663: Finch GR, et al. Comparison of Giardia lamblia and Giardia muris cyst inactivation by ozone. Appl. Environ. Microbiol. 59: 3674-3680, 1993. PubMed: 8285675 32936: Swarbrick A, et al. Nucleotide variaation in the cytidine triphosphate synthetase gene of Giardia duodenalis. J. Eukaryot. Microbiol. 44: 531-534, 1997. PubMed: 9435124 37722: Madico G, et al. Diagnosis of Trichomonas vaginalis infection by PCR using vaginal swab samples. J. Clin. Microbiol. 36: 3205-3210, 1998. PubMed: 9774566 70387: Ey PL, Darby JM. VSP417-6, a variant-specific surface protein encoded at a sixth locus within the vsp417 gene subfamily of Giardia intestinalis. Int. J. Parasitol. 32: 425-436, 2002. PubMed: 11849639 70793: Karanis P, Ey PL. Characterization of axenic isolates of Giardia intestinalis established from humans and animals in Germany. Parasitol. Res. 84: 442-449, 1998. PubMed: 9660132
Isoenzyme electrophoresis [5021] not detected by Trichomonas vaginalis-specific PCR primers [37722] Respiratory metabolism [4977] Equivalency of two nuclei [4839] Factors influencing attachment of flagellate [21702] Excretory/secretory products [217
ATCC30888
