ATCC® Number:30872™
Organism: Acanthamoeba polyphaga (Puschkarew) Page
Designations: CCAP 1501/3b
Isolation: freshwater, Tuskegee, AL, 1965
Depositors: CCAP
History: ATCC<<--CCAP<<--F.C. Page 45
Biosafety Level:1
Shipped: frozen
Growth Conditions: ATCCmedium 712: PYGTemperature: 25.0°C Growth condition: Axenic Protocol: This strain is distributed as a frozen preparation. See the general procedures for thawing a frozen vial. Once vial is thawed, aseptically transfer the entire contents to a 16 X 125 mm screw-capped test tube or a T-25 tissue culture flask containing 5.0 ml of ATCCmedium 712. Incubate horizontally at 25C. Trophozoites should be evident in 1-5 days.
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Comments: Saprozoic growth of Mycobacterium avium in coculture [34879] phylogeny [24028]
Classification: KINGDOM: Protozoa
References: 4757: Page FC. Re-definition of the genus Acanthamoeba with descriptions of three species. J. Protozool. 14: 709-724, 1967. PubMed: 5604481 23896: Daggett PM, et al. Distribution and possible interrelationships of pathogenic and nonpathogenic Acanthamoeba from aquatic environments. Microb. Ecol. 8: 371-386, 1982. 24028: Daggett PM, et al. A molecular approach to the phylogeny of Acanthamoeba. Biosystems 18: 399-405, 1985. PubMed: 4084681 34879: Steinert M, et al. Mycobacterium avium bacilli grow saprozoically in coculture with Acanthamoeba polyphaga and survive within cyst walls. Appl. Environ. Microbiol. 64: 2256-2261, 1998. PubMed: 9603844 70201: Alves JMP, et al. Random amplified polymorphic DNA probes as a tool for the characterization of Brazilian keratitis isolates of the genus Acanthamoeba. Braz. J. Med. Biol. Res. 33: 19-26, 2000.
