ATCC? Number:30841?
Organism: Acanthamoeba lenticulata Molet and Ermolieff-Braun
Designations: PD2S [AC-006]
Isolation: reisolated after passage through mouse, swimming pool, Strasbourg, France, 1976
Depositors: B Molet
Biosafety Level:1
Shipped: freeze-dried
Growth Conditions: ATCCmedium 712: PYGTemperature: 25.0°C Duration: axenic Protocol: ATCCNO: 30010 SPEC: This strain is distributed as a freeze-dried preparation. See the general procedures for opening a freeze-dried vial. Aseptically add 0.5 ml of ice cold medium containing 12% (w/v) sucrose to the freeze-dried inner shell vial. Once the culture is completely rehydrated, aseptically add 1 ml of ATCCmedium 712 and distribute to a 16 X 125 mm plastic screw-capped test tube or a T-25 tissue culture flask containing 5.0 ml of the same medium. Incubate the test tube culture horizontally with the cap on tight. Trophozoites should be evident in 1-5 days.
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Comments: Taxonomy based on isoenzyme profiles and rDNA PCR-RFLP patterns [32205] description [4940] species description [4831] review [23576] Molecular characterization of corneal pathogen [23850] phylogeny [24028] Group I introns [4726]
Cross References: Nucleotide (GenBank) : U02539 Acanthamoeba lenticulata PD2S ATCC# 30841 nuclear small subunit ribosomal DNA group I intron.
Classification: KINGDOM: Protozoa
References: 4726: Gast RJ, et al. Discovery of group I introns in the nuclear small subunit ribosomal RNA genes of Acanthamoeba. Nucleic Acids Res. 22: 592-596, 1994. PubMed: 8127708 4831: Moura H, et al. Acanthamoeba healyi n. sp. and the isoenzyme and immunoblot profiles of Acanthamoeba spp., groups 1 and 3. J. Protozool. 39: 573-583, 1992. PubMed: 1522539 4940: Molet and Ernolieff-Braun. Description d'une amibe d'eau douce: Acanthamoeba lenticulata, sp. nov. (amoebida). Protistologica 12: 571-576, 1976. 23576: John DTOpportunistically pathogenic free-living amebaeIn: John DTParasitic protozoa2nd ed.3San DiegoAcademic Presspp. 143-246, 1993 23850: Ledee DR, et al. Acanthamoeba griffini, molecular characterization of a new corneal pathogen. Invest. Ophthalmol. Vis. Sci. 37: 544-550, 1996. PubMed: 8595954 23896: Daggett PM, et al. Distribution and possible interrelationships of pathogenic and nonpathogenic Acanthamoeba from aquatic environments. Microb. Ecol. 8: 371-386, 1982. 24028: Daggett PM, et al. A molecular approach to the phylogeny of Acanthamoeba. Biosystems 18: 399-405, 1985. PubMed: 4084681 32162: Stothard DR, et al. The evolutionary history of the genus Acanthamoeba and the identification of eight new 18S rRNA gene sequence types. J. Eukaryot. Microbiol. 45: 45-54, 1998. PubMed: 9495032 32205: Kim YH, et al. Close relatedness of Acanthamoeba pustulosa with Acanthamoeba palestinensis based on isoenzyme profiles and rDNA PCR-RFLP patterns. Korean J. Parasitol. 34: 259-266, 1996. PubMed: 9017912 43732: Stothard DR, et al. Fluorescent oligonucleotide probes for clinical and environmental detection of Acanthamoeba and the T4 18S rRNA gene sequence type. J. Clin. Microbiol. 37: 2687-2693, 1999. PubMed: 10405422 52568: Schroeder JM, et al. Use of subgenic 18s ribosomal dna pcr and sequencing for genus and genotype identification of acanthamoebae from humans with keratitis and from sewage sludge. J. Clin. Microbiol. 39: 1903-1911, 2001. PubMed: 11326011 70181: Gast RJ. Development of an Acanthamoeba-specific reverse dot-blot and the discovery of a new ribotype. J. Eukaryot. Microbiol. 48: 609-615, 2001. PubMed: 11831768 70302: Schroeder-Diedrich JM, et al. Group-I introns with unusual sequences occur at three sites in nuclear 18S rRNA genes of Acanthamoeba lenticulata. Curr. Genet. 34: 71-78, 1998. PubMed: 9683678 70805: Ledee DR, et al. Advantages of using mitochondrial 16S rDNA sequences to classify clinical isolates of Acanthamoeba. Invest. Ophthalmol. Vis. Sci. 44: 1142-1149, 2003. PubMed: 12601042 71069: Marciano-Cabral F, Cabral G. Acanthamoeba spp. as agents of disease in humans. Clin. Microbiol. Rev. 16: 273-307, 2003. PubMed: 12692099 89071: Flint JA, et al. Genetic analysis of forty isolates of Acanthamoeba Group III by multilocus isoenzyme electrophoresis. Acta Protozool. 42: 317-324, 2003.