ATCC? Number:30817?
Organism: Crithidia luciliae thermophila Roitman et al.
Isolation: Zelus leucogrammas, Goiania, Brazil, 1977
Depositors: I Roitman
History: ATCC<<--I Roitman<<--M. Deane <<--- A.L.M. Carvalho
Biosafety Level:1
Shipped: frozen
Growth Conditions: ATCCmedium 355: Crithidia mediumMax Temperature: 37.0°C Min Temperature: 25.0°C Duration: axenic Protocol: ATCCNO: 11745 SPEC: See general instructions for thawing and storage of frozen material before proceeding. Add thawed contents to a single 16 x 125 mm glass screw-capped test tube of the appropriate medium. Incubate the culture vertically with the cap screwed on tightly. It is essential to establish cultures initially in small volumes. Once established, the culture can be scaled up to larger volumes. Vigorously agitate the culture and aseptically transfer 0.1 ml of culture to a fresh tube of medium weekly.
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Type Strain: yes(type strain)
Comments: Growth at high temperature [4791] Trypanosomatids from fruit [4818] Riboprinting and taxonomy [23607] Thermal regulation of oxygen-scavenging enzymes [23820] Monoclonal antibodies for identification [23826] endonuclease-generated fragments of K-DNA, esterase isoenzymes, surface proteins for species identification [24216] Multiple distinct site-specific elements in miniexon arrays [24177] Cyclopropane fatty acid [24043] effect of temperature and osmolarity on growth [30421]
References: 4791: Roitman. Growth of Crithidia at high temperature: Crithidia hutneri sp. n. and Crithidia luciliae thermophila s. sp. n.. J. Protozool. 24: 553-556, 1977. 4818: Conchon I, et al. Trypanosomatids, other than Phytomonas spp., isolated and cultured from fruit. J. Protozool. 36: 412-414, 1989. 4950: Gannon JT, Linke HA. Growth studies on xenic cultures of Entamoeba gingivalis using established media. Int. J. Parasitol. 19: 835-838, 1989. PubMed: 2635159 23607: Clark CG. Riboprinting: A tool for the study of genetic diversity in microorganisms. J. Eukaryot. Microbiol. 44: 277-283, 1997. PubMed: 9225441 23820: Emtage MA, Bremner TA. Thermal regualtion of active oxygen-scavenging enzymes in Crithidia Luciliae thermophila. J. Parasitol. 79: 809-814, 1993. 23824: Goncanlves De Lima VM, et al. Comparison of six isoenzymes from 10 species of Crithidia. J. Protozool. 29: 397-401, 1982. 23826: Teixeira MM, Camargo EP. Monoclonal antibodies for the identification of trypanosomatids of the genus Phytomonas. J. Protozool. 36: 262-264, 1989. 24043: Fish WR, et al. The cyclopropane fatty acid of trypanosomatids. Mol. Biochem. Parasitol. 3: 103-115, 1981. PubMed: 7254247 24177: Teng SC, et al. A new non-LTR retrotransposon provides evidence for multiple distinct site-specific elements in Crithidia fasciculata miniexon arrays. Nucleic Acids Res. 23: 2929-2936, 1995. PubMed: 7659515 24216: Camargo EP, et al. Electrophoretic analysis of endonuclease-generated fragments of k-DNA, of esterase isoenzymes, and of surface proteins as aids for species identification of insect trypanosomatids. J. Protozool. 29: 251-258, 1982. PubMed: 6284925 30421: Da Silva JB, Roitman I. Effect of temperature and osmolarity on growth of Crithidia fasciculata, Crithidia hutneri, Crithidia thermophila, and Herpetomonas samuelpessoai. J. Eukaryot. Microbiol. 29: 269-272, 1982. 34222: Cho J, Eichinger D. Crithidia fasciculata induces encystation of Entamoeba invadens in a galactose-dependent manner. J. Parasitol. 84: 705-710, 1998. PubMed: 9714198