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平臺編號：bio-48097
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國際編號：ATCC 30811
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拉丁屬名： Naegleria lovaniensis
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規(guī)格：frozen
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用途：ATCC原裝進口
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服務費用：
加載中…… - 訂購
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注意事項：僅用于科學研究或者工業(yè)應用等非醫(yī)療目的不可用于人類或動物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準）
Naegleria lovaniensis
Naegleria lovaniensis Steven et al. 拉丁名
(ATCC? 30811?)統(tǒng)一編號
Deposited As Naegleria fowleri Carter Strain Designations LvH1
Biosafety Level 生物安全等級 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. Isolation thermally polluted canal, Belgium, 1974
Product Format 提供形式 frozen
Type Strain 模式菌株 no
Comments 注釋 Comparative study of Naegleria strains；
Axenic medium for differentiation of pathogenic species；
isoenzyme patterns of pathogenic and non-pathogenic spp.；
Isoenzyme identification of pathogenic species；
Medium 培養(yǎng)基 ATCC? Medium 997: Fresh water ameba medium
Growth Conditions 生長條件
Temperature 培養(yǎng)溫度: 35.0°C
Growth condition: grown with Citrobacter freundii and mixed bacteria
Cryopreservation 1. Allow the cells to encyst. To detach cysts from the plate flush the surface with 5 ml fresh ATCC? medium 1323 (Page's Balanced Salt Solution). Rub the surface of the plate with a spread bar to detach adhering amoebae. 2. 2， Transfer the cyst suspension to a sterile centrifuge tube.
3. If the cyst concentration does not exceed 2 x 106 cysts/ml adjust the suspension to that concentration. To adjust the concentration, centrifuge at 600 x g for 5 min and resuspend the pellet in the volume of fresh medium required to yield 2 x 106.
4. , While cells are centrifuging prepare a 15% (v/v) solution of sterile DMSO as follows: Add the required volume of DMSO to a glass screw-capped test tube and place it in an ice bath. Allow the DMSO to solidify. Add the required volume of refrigerated medium. Dissolve the DMSO by inverting the tube several times.
Name of Depositor 寄存人 JF De Jonckheere
Year of Origin 收藏年份 1974
References 參考文獻 De Jonckheere J. Use of an axenic medium for differentiation between pathogenic and nonpathogenic Naegleria fowleri isolates. Appl. Environ. Microbiol. 33: 751-757, 1977. PubMed: 869525
De Jonckheere J, van de Voorde H. Comparative study of six strains of Naegleria with special reference to nonpathogenic variants of Naegleria fowleri. J. Protozool. 24: 304-309, 1977. PubMed: 881654
De Jonckheere JF. Isoenzyme patterns of pathogenic and non-pathogenic Naegleria spp. using agarose isoelectric focusing. Ann. Microbiol. 133: 319-342, 1982.
De Jonckheere JF. Isoenzyme patterns of pathogenic and non-pathogenic Naegleria spp. using agarose isoelectric focusing. Ann. Microbiol. (Paris) 133: 319-342, 1982. PubMed: 6211120
De Jonckheere JF. A century of research on the amoeboflagellate genus Naegleria. Acta Protozool. 41: 309-342, 2002.
