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WHO Israel LRC 146 Leishmania tropica (Wright) Luhe 菌株拉丁名
(ATCC? 30816?) 菌株編號(hào)
Deposited As Leishmania adleri
Strain Designations 菌株別名 WHO Israel LRC 146
Application 用途 Vector borne research
Biosafety Level 生物安全等級(jí) 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Isolation 分離源 lizard, Latastia longicauda revoili, Kenya
Product Format 提供形式 frozen
Type Strain 模式菌株 no
Comments 注釋 Taxonomy (ATCC lab.). Promastigotes. In vitro phagocytosis by lizard leukocytes Taxonomic status Variation in kinetoplast minicircle and maxicircle DNA sequences Cyclopropane fatty acid
Medium 培養(yǎng)基 ATCC? Medium 811: Leishmania medium
Growth Conditions 生長(zhǎng)條件
Temperature 培養(yǎng)溫度 : 25.0°C
Duration: axenic
Protocol: ATCC
NO: 11745
SPEC: See general instructions for thawing and storage of frozen material before proceeding. Add thawed contents to a single 16 x 125 mm glass screw-capped test tube of the appropriate medium.
Incubate the culture vertically with the cap screwed on tightly. It is essential to establish cultures initially in small volumes. Once established, the culture can be scaled up to larger volumes.
Vigorously agitate the culture and aseptically transfer 0.1 ml of culture to a fresh tube of medium weekly.
Subcultivation Protocol: ATCC
NO: 11745
SPEC: See general instructions for thawing and storage of frozen material before proceeding.
Add thawed contents to a single 16 x 125 mm glass screw-capped test tube of the appropriate medium. Incubate the culture vertically with the cap screwed on tightly. It is essential to establish cultures initially in small volumes.
Once established, the culture can be scaled up to larger volumes. Vigorously agitate the culture and aseptically transfer 0.1 ml of culture to a fresh tube of medium weekly.
Name of Depositor 寄存人 J Janovy
Chain of Custody 來(lái)源國(guó)家 ATCC <<--J Janovy<<--WHO LRC Jerusalem <<--- . . . <<--- Heisch?
References 參考文獻(xiàn) Dollahon NR, Janovy J Jr.. Leishmania adleri: in vitro phagocytosis by lizard leukocytes and peritoneal exudate cells. Exp. Parasitol. 34: 56-61, 1973. PubMed: 4722485
Dollahon NR, Janovy J Jr.. Experimental infection of New World lizards with Old World lizard Leishmania species. Exp. Parasitol. 36: 253-260, 1974. PubMed: 4413377
Simpson L. The status of Leishmania tarentolae/Trypanosoma platydactyli. Parasitol. Today 4: 115-118, 1988. Fish WR, et al. The cyclopropane fatty acid of trypanosomatids. Mol. Biochem. Parasitol. 3: 103-115, 1981. PubMed: 7254247
Gomez-Eichelmann MC, et al. Comparison of several lizard Leishmania species and strains in terms of kinetoplast minicircle and maxicircle DNA sequences, nuclear chromosomes, and membrane lipids [published erratum appears in Mol. Biochem. Parasitol. 28: 301, 1988]. Mol. Biochem. Parasitol. 27: 143-158, 1988. PubMed: 3344003