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平臺編號：bio-48103
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國際編號：ATCC 30810
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拉丁屬名： Naegleria fowleri Carter
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規(guī)格：frozen
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用途：ATCC原裝進口
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服務(wù)費用：
加載中…… - 訂購
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注意事項：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準）
Naegleria fowleri Carter
Naegleria fowleri Carter 拉丁名
(ATCC? 30810?) 統(tǒng)一編號
Strain Designations 別名 75/36/S
Biosafety Level 生物安全等級 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. Isolation mud from thermally polluted stream, Belgium, 1975
Product Format 提供形式 frozen
Type Strain 模式菌種 no
Comments 注釋 acis phosphatase and leucine amino peptidase activity for identification Distribution in man-made thermal waters isoenzyme patterns of pathogenic and non-pathogenic spp.
Medium 培養(yǎng)基 ATCC? Medium 1034: Modified PYNFH medium (Available from ATCC as ATCC cat. no. 327-X)
Growth Conditions 生長條件
Temperature 培養(yǎng)溫度: 35.0°C
Duration: axenic
Cryopreservation 1. Harvest cells from a culture that is at or near peak density by centrifugation at 600 x g for 5 min. Pool the cell pellets into a single tube.
2. Adjust the concentration of cells to 2.0 x 106/ml. If the concentration is too low, centrifuge at 600 x g for 5 minutes and resuspend the cell pellet with a volume of supernatant to yield the desired concentration.
3. Prepare a 15% (v/v) sterile DMSO solution in ATCC medium 1034 as follows: Add the required volume of DMSO to a glass screw-capped test tube and place on ice. Allow the DMSO to solidify. Add the required volume of refrigerated ATCC medium 1034. Dissolve the DMSO by inverting several times. If the DMSO solution is not prepared on ice, an exothermic reaction will occur that may precipitate certain components of the medium.
4. Mix the cell preparation and the DMSO in equal portions. Thus, the final concentration will be 106 and 7.5% (v/v) DMSO. The time from the mixing of the cell preparation and DMSO stock solution before the freezing process is begun should be no less than 15 min and no longer than 60 min.
5. Dispense in 0.5 ml aliquots into 1.0 - 2.0 ml sterile plastic screw-capped cryules (special plastic vials for cryopreservation). 6. Place vials in a controlled rate freezing unit. From room temperature cool at -1°C/min to -40°C. If freezing unit can compensate for the heat of fusion, maintain rate at -1 C/min through heat of fusion. At -40°C plunge the ampules into liquid nitrogen.
7. The frozen preparations are stored in either the vapor or liquid phase of a nitrogen refrigerator.
8. To establish a culture from the frozen state place an ampule in a water bath set at 35°C. Immerse the vial enough to cover only the frozen material. Do not agitate the vial.
9. Immediately after thawing, do not leave in the water bath, aseptically remove the contents of the ampule and inoculate into 5.0 ml of fresh ATCC medium 1034.
10. Incubate the tube on a 15° horizontal at 35°C with the cap screwed on tightly.
Name of Depositor 寄存人 JF De Jonckheere
Year of Origin 收藏年份 1975
References de Jonckheere J, Voorde H. The distribution of Naegleria fowleri in man-made thermal waters. Am. J. Trop. Med. Hyg. 26: 10-15, 1977. PubMed: 842770
De Jonckheere J. Use of an axenic medium for differentiation between pathogenic and nonpathogenic Naegleria fowleri isolates. Appl. Environ. Microbiol. 33: 751-757, 1977. PubMed: 869525
De Jonckheere JF, Dierickx PJ. Determination of acid phosphatase and leucine amino peptidase activity as an identification method for pathogenic Naegleria fowleri. Trans. R. Soc. Trop. Med. Hyg. 76: 773-775, 1982. PubMed: 7164144
De Jonckheere JF. Isoenzyme patterns of pathogenic and non-pathogenic Naegleria spp. using agarose isoelectric focusing. Ann. Microbiol. (Paris) 133: 319-342, 1982. PubMed: 6211120
