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Naegleria fowleri Carter 拉丁名

(ATCC? 30808?) 統(tǒng)一編號(hào)

Strain Designations 菌種別名 KUL 

Biosafety Level 生物安全等級(jí) 2 

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. 

Isolation 分離基物 human cerebrospinal fluid, Belgium, 1973 

Product Format 提供形式 frozen 

Type Strain 模式菌株 no 

Comments Isoenzyme identification of species； 

Occurrence in aquaria； 

Axenic medium for differentiation of pathogenic species； 

acis phosphatase and leucine amino peptidase activity for Identification； 

Comparative study of Naegleria strains； 

Distribution in man-made thermal waters； 

isoenzyme patterns of pathogenic and non-pathogenic spp.； 

Interrepeat PCR； Case report； 

Comparative biochemical study； 

Occurence in natural thermal waters in Mexico；

Medium 培養(yǎng)基 ATCC? Medium 1034: Modified PYNFH medium (Available from ATCC as ATCC cat. no. 327-X) 

Growth Conditions 生長(zhǎng)條件

Temperature 培養(yǎng)溫度: 35.0°C 

Duration: axenic 

Cryopreservation 1.  Harvest cells from a culture that is at or near peak density by centrifugation at 600 x g for 5 min. Pool the cell pellets into a single tube.

2.  Adjust the concentration of cells to 2.0 x 106/ml.  If the concentration is too low, centrifuge at 600 x g for 5 minutes and resuspend the cell pellet with a volume of supernatant to yield the desired concentration. 

3.   Prepare a 15% (v/v) sterile DMSO solution in ATCC medium 1034 as follows:  Add the required volume of DMSO to a glass screw-capped test tube and place on ice.  Allow the DMSO to solidify.  Add the required volume of refrigerated ATCC medium 1034. Dissolve the DMSO by inverting several times.  If the DMSO solution is not prepared on ice, an exothermic reaction will occur that may precipitate certain components of the medium. 

4.  Mix the cell preparation and the DMSO in equal portions. Thus, the final concentration will be 106 and 7.5% (v/v) DMSO. The time from the mixing of the cell preparation and DMSO stock solution before the freezing process is begun should be no less than 15 min and no longer than 60 min. 

5.  Dispense in 0.5 ml aliquots into 1.0 - 2.0 ml sterile plastic screw-capped cryules (special plastic vials for cryopreservation). 

6.  Place vials in a controlled rate freezing unit. From room temperature cool at -1°C/min to -40°C. If freezing unit can compensate for the heat of fusion, maintain rate at -1 C/min through heat of fusion. At -40°C plunge the ampules into liquid nitrogen. 

7.  The frozen preparations are stored in either the vapor or liquid phase of a nitrogen refrigerator. 

8.  To establish a culture from the frozen state place an ampule in a water bath set at 35°C. Immerse the vial enough to cover only the frozen material. Do not agitate the vial. 

9.  Immediately after thawing, do not leave in the water bath, aseptically remove the contents of the ampule and inoculate into 5.0 ml of fresh ATCC medium 1034. 

10.  Incubate the tube on a 15° horizontal at 35°C with the cap screwed on tightly.