ATCC® Number:30809™
Organism: Naegleria fowleri Carter
Designations: 76/14/S3
Isolation: mud from thermally polluted canal, Belgium, 1976
Depositors: JF De Jonckheere
Biosafety Level:2
Shipped: frozen
Growth Conditions: ATCCmedium 1034: Modified PYNFH medium (Available from ATCCas ATCCcat. no. 327-X)Temperature: 35.0°C Duration: axenic
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Comments: isoenzyme patterns of pathogenic and non-pathogenic spp. [23838] Group I intron in the SSUrDNA [23861] acis phosphatase and leucine amino peptidase activity for identification [4923]
References: 4657: De Jonckheere J. Use of an axenic medium for differentiation between pathogenic and nonpathogenic Naegleria fowleri isolates. Appl. Environ. Microbiol. 33: 751-757, 1977. PubMed: 869525 4923: De Jonckheere JF, Dierickx PJ. Determination of acid phosphatase and leucine amino peptidase activity as an identification method for pathogenic Naegleria fowleri. Trans. R. Soc. Trop. Med. Hyg. 76: 773-775, 1982. PubMed: 7164144 23838: De Jonckheere JF. Isoenzyme patterns of pathogenic and non-pathogenic Naegleria spp. using agarose isoelectric focusing. Ann. Microbiol. (Paris) 133: 319-342, 1982. PubMed: 6211120 23861: De Jonckheere JF. A Group I intron in the SSUrDNA of some Naegleria spp. demonstrated by polymerase chain reaction amplification. J. Eukaryot. Microbiol. 40: 179-187, 1993. PubMed: 8461891
