ATCC® Number:30807™
Organism: Acanthamoeba polyphaga (Puschkarew) Page
Designations: MC-1
Isolation: spleen of Micropterus coosai, Chinnabee Lake, AL, 1974
Depositors: PW Taylor
Biosafety Level:2
Shipped: dried
Growth Conditions: ATCCmedium 997: Fresh water ameba mediumTemperature: 25.0°C Duration: grown with bacteria Protocol: ATCCNO: 30011 SPEC: This strain is distributed as a dried preparation. See the general procedures for opening a dried vial. Aseptically add 1 ml of sterile distilled water to the inner shell vial, remove the filter paper aseptically with a pair of forceps, and place it in the center of an agar plate of ATCCmedium 997. Add the liquid remaining in the vial to the plate and spread it evenly over the surface of the plate. Incubate the plate at 25C. Trophozoites (amebae) should be evident within 2-3 days.
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Comments: Identical to Acanthamoeba polyphaga ATCC30173 on the basis of starch gel electrophoretic analysis of enzymes. Infection in freshwater fishes [4974] phylogeny [24028]
Classification: KINGDOM: Protozoa
References: 4974: Taylor PW. Isolation and experimental infection of free-living amebae in freshwater fishes. J. Parasitol. 63: 232-237, 1977. PubMed: 870664 23896: Daggett PM, et al. Distribution and possible interrelationships of pathogenic and nonpathogenic Acanthamoeba from aquatic environments. Microb. Ecol. 8: 371-386, 1982. 24028: Daggett PM, et al. A molecular approach to the phylogeny of Acanthamoeba. Biosystems 18: 399-405, 1985. PubMed: 4084681
ATCC30807
