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平臺(tái)編號(hào)：bio-48164
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國(guó)際編號(hào)：ATCC 30772
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拉丁屬名： Crypthecodinium cohnii
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規(guī)格：frozen
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用途：ATCC原裝進(jìn)口
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服務(wù)費(fèi)用：
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注意事項(xiàng)：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類(lèi)或動(dòng)物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫(kù)為準(zhǔn)）
隱甲藻
Crypthecodinium cohnii (Seligo) Javornicky 拉丁名
(ATCC? 30772?) 統(tǒng)一編號(hào)
Strain Designations 菌種別名 PGM-1
Application 用途 Biofuel production
Biosafety Level 生物安全等級(jí) 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Isolation 分離基物 Macrocystis sp., Pacific Grove, CA, 1977
Product Format 提供形式 frozen
Storage Conditions 保藏條件
Frozen 冷凍物: -70°C or colder
Freeze-Dried 凍干物: 2°C to 8°C
Live Culture: See Protocols Section
Type Strain 模式菌株 no
Comments 注釋 Major Sibling Species WH Geographical distribution Sexuality and meiosis
Medium 培養(yǎng)基 ATCC? Medium 460: A2E6 medium
Growth Conditions 生長(zhǎng)條件
Temperature 培養(yǎng)溫度: 20°C to 25°C
Culture System: Axenic
Cryopreservation 1，Harvest cells from cultures which are at or near peak density. Aseptically transfer cells to 15 mL plastic centrifuge tubes and centrifuge at ~150 x g for 5 min.,
2，Adjust the concentration of cells to 2 x 106/mL with fresh medium, then dilute to half this concentration by adding an equal amount of a 15% (v/v) sterile glycerol solution in fresh medium.,
3，Dispense in 0.5 mL aliquots into 1.0 - 2.0 mL sterile plastic screw-capped cryules (special plastic vials for cryopreservation). The time from mixing of the cell suspension and the glycerol solution, before the cooling cycle begins, should be no greater than 15 min. Place vials in a controlled rate freezing unit. From room temperature cool at -1°C/min to -40°C. If freezing unit can compensate for the heat of fusion, maintain rate at -1 C/min through heat of fusion. At -40°C plunge ampules into liquid nitrogen. Alternatively, place the vials in a Nalgene 1°C freezing apparatus.
4,Place the apparatus at -80°C for 1.5 to 2 hours and then plunge ampules into liquid nitrogen. (The cooling rate in this apparatus is approximately -1°C/min.)
5, The frozen preparations should be stored in either the vapor or liquid phase of a nitrogen refrigerator. Frozen preparations stored below -130°C are stabile indefinitely. Those stored at temperatures above -130°C are progressively less stabile as the storage temperature is elevated. Vials can be stored between -80 and -70°C for no longer than one week.
6,To establish a culture from the frozen state place an ampule in a water bath set at 35°C. Immerse the vial to a level just above the surface of the frozen material. Do not agitate the vial.
7, Immediately after thawing, do not leave in the water bath, aseptically remove the contents of the ampule and transfer to a fresh tube containing 5 mL of ATCC Medium 460. Incubate the tube vertically at 20-25°C with the cap loosened one half turn. Subculture every 10-14d.
Name of Depositor CA Beam, M Himes
Year of Origin 1977
References Beam CA, Himes M. Distribution of members of the Crypthecodinium cohnii (Dinophyceae) species complex. J. Protozool. 29: 8-15, 1982. Beam CA, Himes M. Sexuality and meiosis in dinoflagellates. In: Beam CA, Himes M, Biochemistry and physiology of protozoa, 2nd ed.3 New York Academic Press: 171-206, 1980.
