ATCC® Number:30730™
Organism: Acanthamoeba hatchetti Sawyer et al.
Designations: BH-2
Isolation: sediment, Baltimore Harbor, MD, 1974
Depositors: TK Sawyer
Biosafety Level:2
Shipped: dried
Growth Conditions: ATCCmedium 711: PYBTemperature: 25.0°C Duration: grown with bacteria Protocol: ATCCNO: 30011 SPEC: This strain is distributed as a dried preparation. See the general procedures for opening a dried vial. Aseptically add 1 ml of sterile distilled water to the inner shell vial, remove the filter paper aseptically with a pair of forceps, and place it in the center of an agar plate of ATCCmedium 997. Add the liquid remaining in the vial to the plate and spread it evenly over the surface of the plate. Incubate the plate at 25C. Trophozoites (amebae) should be evident within 2-3 days.
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Type Strain: yes(type strain)
Comments: Amoebas from brackish and ocean sediments [5052] mitochondrial DNA fingerprinting [21722] phylogeny [24028] quantitative bacterial plaque assay for enumeration [24095] characterization of Acanthamoeba polyphaga [24209] review [23576]
Classification: KINGDOM: Protozoa
References: 5052: Sawyer TK, et al. Pathogenic amoebas from brackish and ocean sediments, with a description of Acanthamoeba hatchetti, n. sp.. Science 196: 1324-1325, 1977. PubMed: 867031 21722: Gautom RK, et al. Mitochondrial DNA fingerprinting of Acanthamoeba spp. isolated from clinical and environmental sources. J. Clin. Microbiol. 32: 1070-1073, 1994. PubMed: 7913095 23576: John DTOpportunistically pathogenic free-living amebaeIn: John DTParasitic protozoa2nd ed.3San DiegoAcademic Presspp. 143-246, 1993 23896: Daggett PM, et al. Distribution and possible interrelationships of pathogenic and nonpathogenic Acanthamoeba from aquatic environments. Microb. Ecol. 8: 371-386, 1982. 24028: Daggett PM, et al. A molecular approach to the phylogeny of Acanthamoeba. Biosystems 18: 399-405, 1985. PubMed: 4084681 24095: Anger C, et al. A quantitative bacterial plaque assay for the enumeration of viable Acanthamoeba cells. Rev. Infect. Dis. 13 suppl.5: S396, 1991. PubMed: 2047673 24209: Fritsche TR, et al. Occurrence of bacterial endosymbionts in Acanthamoeba spp. isolated from corneal and environmental specimens and contact lenses. J. Clin. Microbiol. 31: 1122-1126, 1993. PubMed: 8501212 32325: Alizadeh H, et al. In vitro amoebicidal activity of propamidine and pentamidine isethionate against Acanthamoeba species and toxicity to corneal tissues. Cornea 16: 94-100, 1997. PubMed: 8985640 57068: Kong HH, et al. Mitochondrial DNA restriction fragment length polymorphism (RFLP) and 18S small-subunit ribosomal DNA PCR-RFLP analyses of Acanthamoeba isolated from contact lens storage cases of residents in southwestern Korea. J. Clin. Microbiol. 40: 1199-1206, 2002. PubMed: 11923331 71069: Marciano-Cabral F, Cabral G. Acanthamoeba spp. as agents of disease in humans. Clin. Microbiol. Rev. 16: 273-307, 2003. PubMed: 12692099
ATCC30730
