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平臺編號：bio-48175
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國際編號：30727
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拉丁屬名： Heliophrya sp.
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規(guī)格：frozen
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用途：ATCC原裝進口
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服務費用：
加載中…… - 訂購
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注意事項：僅用于科學研究或者工業(yè)應用等非醫(yī)療目的不可用于人類或動物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準）
Heliophrya sp.
ATCC? Number:30727?
Organism: Heliophrya sp.
Designations: PWS
Isolation: Potomac River, Washington, DC, 1974
Depositors: DM Spoon
Biosafety Level:1
Shipped: frozen
Growth Conditions: Temperature: 22.0°C Duration: grown with mixed flora and fauna Protocol: ATCCNO: 30727 SPEC: This strain is distributed as a frozen stabilate. See general instructions for thawing and storage of frozen material before proceeding. Remove the frozen ampoule from the dry ice and transfer directly to a 35C water bath. The food source for this strain is Paramecium tetraurelia ATCC30303 (other strains will probably work equally well), not supplied. Empty the thawed contents of the vial into a petri dish and, if necessary, add distilled water to cover the bottom of the dish. Allow Heliophrya cells to attach to the bottom of the dish (overnight). Feed with a culture of paramecia every 7-21 days. New cultures may be established by: 1) adding a coverslip to the petri dish, allowing larvae to attach, then transferring the coverslip to another petri dish; or 2) transferring adult Heliophrya floating in surface film to a new dish by means of a wire loop.
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Classification: KINGDOM: Protozoa
Organism: Heliophrya sp.
Designations: PWS
Isolation: Potomac River, Washington, DC, 1974
Depositors: DM Spoon
Biosafety Level:1
Shipped: frozen
Growth Conditions: Temperature: 22.0°C Duration: grown with mixed flora and fauna Protocol: ATCCNO: 30727 SPEC: This strain is distributed as a frozen stabilate. See general instructions for thawing and storage of frozen material before proceeding. Remove the frozen ampoule from the dry ice and transfer directly to a 35C water bath. The food source for this strain is Paramecium tetraurelia ATCC30303 (other strains will probably work equally well), not supplied. Empty the thawed contents of the vial into a petri dish and, if necessary, add distilled water to cover the bottom of the dish. Allow Heliophrya cells to attach to the bottom of the dish (overnight). Feed with a culture of paramecia every 7-21 days. New cultures may be established by: 1) adding a coverslip to the petri dish, allowing larvae to attach, then transferring the coverslip to another petri dish; or 2) transferring adult Heliophrya floating in surface film to a new dish by means of a wire loop.
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Classification: KINGDOM: Protozoa
