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ATCC? Number:30732?
Organism: Acanthamoeba jacobsi Sawyer et al.
Designations: 31-B [AC-005]
Isolation: marine sediment, New York Bight Apex, 1974
Depositors: TK Sawyer
Biosafety Level:2
Shipped: frozen
Growth Conditions: ATCCmedium 997: Fresh water ameba mediumTemperature: 25.0°C Duration: grown with bacteria Protocol: ATCCNO: 30011 SPEC: This strain is distributed as a dried preparation. See the general procedures for opening a dried vial. Aseptically add 1 ml of sterile distilled water to the inner shell vial, remove the filter paper aseptically with a pair of forceps, and place it in the center of an agar plate of ATCCmedium 997. Add the liquid remaining in the vial to the plate and spread it evenly over the surface of the plate. Incubate the plate at 25C. Trophozoites (amebae) should be evident within 2-3 days.
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Type Strain: yes [23869]
Comments: species description [23869] phylogeny [24028]
Cross References: Nucleotide (GenBank) : AY26236018S ribosomal RNA gene, partial sequence
Classification: KINGDOM: Protozoa
References: 23869: Sawyer TK, et al. Acanthamoeba jacobsi sp. n. (Protozoa: Acanthamoebidae) from sewage contaminated ocean sediments. J. Helminthol. Soc. Wash. 59: 223-226, 1992. 23896: Daggett PM, et al. Distribution and possible interrelationships of pathogenic and nonpathogenic Acanthamoeba from aquatic environments. Microb. Ecol. 8: 371-386, 1982. 24028: Daggett PM, et al. A molecular approach to the phylogeny of Acanthamoeba. Biosystems 18: 399-405, 1985. PubMed: 4084681 71069: Marciano-Cabral F, Cabral G. Acanthamoeba spp. as agents of disease in humans. Clin. Microbiol. Rev. 16: 273-307, 2003. PubMed: 12692099 89071: Flint JA, et al. Genetic analysis of forty isolates of Acanthamoeba Group III by multilocus isoenzyme electrophoresis. Acta Protozool. 42: 317-324, 2003. 89072: Hewett MK, et al. Identification of a new Acanthamoeba 18S rRNA gene sequence type, corresponding to the species Acanthamoeba jacobsi Sawyer, Nerad and Visvesvara, 1992 (Lobosea: Acanthamoebidae). Acta Protozool. 42: 325-329, 2003.