ATCC? Number:30703?
Organism: Tetramitus jugosus (Page) Brown and De Jonckheere
Designations: CCAP 1588/3a [F. Page 35]
Isolation: stream, Moscow Mountain, ID, 1965
Depositors: CCAP
Biosafety Level:1
Shipped: dried
Growth Conditions: ATCCmedium 711: PYBATCCmedium 997: Fresh water ameba mediumMax Temperature: 25.0°C Min Temperature: 20.0°C Duration: grown with Escherichia coli ATCC11775 Protocol: ATCCNO: 30133 SPEC: This strain is shipped as a dried preparation. Aseptically add 1 ml of sterile distilled water to the inner vial, remove the filter paper pellet with a pair of forceps, and place it in the center of an agar plate of ATCCmedium 711 or 997. Add the liquid remaining in the vial to the plate and spread it evenly over the surface of the plate. Incubate the plate at 25C. Trophozoites (amebae) should be evident within 2-3 days.
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Type Strain: yes(type strain)
Comments: strain description [23872] biochemical identification [4799]
Cross References: Nucleotide (GenBank) : M98050 Paratetramitus jugosus 16S ribosomal RNA-like gene.
References: 4799: Daggett PM, Nerad TA. The biochemical identification of vahlkampfiid amoebae. J. Protozool. 30: 126-128, 1983. PubMed: 6864593 4939: Darbyshire. . Protistologica 12: 375-387, 1976. 23798: Pandey VS. Effect of temperature on development of the free-living stages of Ostertagia ostertagi. J. Parasitol. 58: 1037-1041, 1972. PubMed: 4641869 23872: Read LK, et al. A new strain of Paratetramitus jugosis from Laguna Figueroa, Baja California, Mexico. Biol. Bull. 165: 241-264, 1983.