ATCC? Number:30704?
Organism: Tetramitus jugosus (Page) Brown and De Jonckheere
Designations: CCAP 1588/3b, Lingo
Isolation: soil, Orkney Islands, United Kingdom, 1973
Depositors: CCAP 
History: ATCC<<--CCAP<<--J.F. Darbyshire
Biosafety Level:2
Shipped: freeze-dried 
Growth Conditions: ATCCmedium 711: PYBMax Temperature: 25.0°C Min Temperature: 20.0°C Duration: grown with Escherichia coli ATCC11775 Protocol: ATCCNO: 30133 SPEC: This strain is shipped as a dried preparation. Aseptically add 1 ml of sterile distilled water to the inner vial, remove the filter paper pellet with a pair of forceps, and place it in the center of an agar plate of ATCCmedium 711 or 997. Add the liquid remaining in the vial to the plate and spread it evenly over the surface of the plate. Incubate the plate at 25C. Trophozoites (amebae) should be evident within 2-3 days.
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Comments: biochemical identification  [4799]
References:  4799: Daggett PM, Nerad TA. The biochemical identification of vahlkampfiid amoebae.  J. Protozool. 30: 126-128, 1983. PubMed: 6864593 4939: Darbyshire. .  Protistologica 12: 375-387, 1976.