ATCC? Number:30641?
Organism: Trypanosoma neveulemairei Brumpt
Isolation: frog, Rana esculenta (imported), Yugoslavia (Univ. Minnesota), 1975
Depositors: FG Wallace
Biosafety Level:1
Shipped: frozen
Growth Conditions: ATCCmedium 947: L diphasic blood agar (ATCCMedium 1011) with Locke's solution overlay reduced from 3.0 ml to 1.0 mlTemperature: 25.0°C Duration: axenic Protocol: ATCCNO: 11745 SPEC: See general instructions for thawing and storage of frozen material before proceeding. Add thawed contents to a single 16 x 125 mm glass screw-capped test tube of the appropriate medium. Incubate the culture vertically with the cap screwed on tightly. It is essential to establish cultures initially in small volumes. Once established, the culture can be scaled up to larger volumes. Vigorously agitate the culture and aseptically transfer 0.1 ml of culture to a fresh tube of medium weekly.
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Comments: Phylogenetic relationships among anuran trypanosomes [4677] Karyotype analysis of anuran trypanosomes [24051]
Classification: KINGDOM: Protozoa
References: 4677: Clark CG, et al. Phylogenetic relationship among anuran Trypanosoma as revealed by riboprinting. J. Eukaryot. Microbiol. 42: 92-96, 1995. PubMed: 7728144 23730: Wallace FG. What is Trypanosoma rotatorium?. J. Protozool. 22: 18A 35, 1975. 24051: Lun ZR, Desser SS. Karyotype analysis of anuran trypanosomes by pulsed-field gradient gel electrophoresis. J. Parasitol. 81: 1018-1020, 1995. PubMed: 8544044