ATCC? Number:30598?
Organism: Herpetomonas sp.
Isolation: human, female liver biopsy, Corpus Christi, TX, 1976
Depositors: RB McGhee
History: ATCC<<--RB McGhee<<--CDC <<--- Spohn Hospital
Biosafety Level:2
Shipped: frozen
Growth Conditions: ATCCmedium 1011: Diphasic blood agar mediumATCCmedium 947: L diphasic blood agar (ATCCMedium 1011) with Locke's solution overlay reduced from 3.0 ml to 1.0 mlMax Temperature: 37.0°C Min Temperature: 25.0°C Duration: axenic Protocol: ATCCNO: 11745 SPEC: See general instructions for thawing and storage of frozen material before proceeding. Add thawed contents to a single 16 x 125 mm glass screw-capped test tube of the appropriate medium. Incubate the culture vertically with the cap screwed on tightly. It is essential to establish cultures initially in small volumes. Once established, the culture can be scaled up to larger volumes. Vigorously agitate the culture and aseptically transfer 0.1 ml of culture to a fresh tube of medium weekly.
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Comments: Unique no-branched polyunsaturated fatty acids [24089] Cyclopropane fatty acid [24043]
References: 5108: McGhee RB, Cosgrove WB. Biology and physiology of the lower Trypanosomatidae. Microbiol. Rev. 44: 140-173, 1980. PubMed: 6997722 24043: Fish WR, et al. The cyclopropane fatty acid of trypanosomatids. Mol. Biochem. Parasitol. 3: 103-115, 1981. PubMed: 7254247 24089: Fish WR, et al. Some Phytomonas and Herpetomonas species form unique iso-branched polyunsaturated fatty acids. Mol. Biochem. Parasitol. 5: 1-18, 1982. PubMed: 7062937