ATCC? Number:30574?
Organism: Paramecium primaurelia Sonneborn
Designations: stock 60
Isolation: Shelburne Pond, Burlington, VT, pre-1941
Depositors: TM Sonneborn
History: ATCC<<--TM Sonneborn<<--V. Tartar
Biosafety Level:1
Shipped: test tube
Growth Conditions: ATCCmedium 802: Sonneborn's Paramecium mediumMax Temperature: 31.0°C Min Temperature: 13.0°C Protocol: ATCCNO: 30300 SPEC: This strain is shipped as a growing test tube culture. Upon arrival, remove test tube from sealed plastic envelope, remove plastic seal from cap, and loosen the cap one half turn. Add 1.0 ml of ATCCmedium 802 bacterized with Enterobacter aerogenes ATCC13048 twice weekly. When the tube is filled to within one inch of the top, decant leaving 5.0 ml in the original tube. Subcultures are established by transferring 0.5 ml of a growing culture to 5.0 ml of bacterized ATCCmedium 802 in a 20 x 120 mm test tube.
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Type Strain: yes(type strain)
Comments: This stock expresses both mating types. Variable telomeric DNA repeats [23906]
Classification: KINGDOM: Protozoa
References: 5004: Sonneborn TM. The Paramecium aurelia complex of fourteen sibling species. Trans. Am. Microsc. Soc. 94: 155-178, 1975. 23906: McCormick-Graham M, Romero DP. A single telomerase RNA is sufficient for the synthesis of variable telomeric DNA repeats in ciliates of the genus Paramecium. Mol. Cell. Biol. 16: 1871-1879, 1996. PubMed: 8657163