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平臺(tái)編號(hào)：bio-48326
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國(guó)際編號(hào)：ATCC 30569
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拉丁屬名： Naegleria lovaniensis
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規(guī)格：frozen
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用途：ATCC原裝進(jìn)口
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注意事項(xiàng)：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類(lèi)或動(dòng)物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫(kù)為準(zhǔn)）
Naegleria lovaniensis
Naegleria lovaniensis Steven et al.拉丁名
(ATCC? 30569?) 統(tǒng)一編號(hào)
Deposited As Naegleria gruberi Schardinger Strain Designations TS
Biosafety Level 生物安全等級(jí) 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. Isolation Vero cell culture 120th passage, Bowling Green, OH, 1970 Product Format frozen
Storage Conditions 保藏條件
Frozen 冷凍物: -80°C or colder
Freeze-Dried 凍干物: 2°C to 8°C
Live Culture: See Propagation Section
Type Strain 模式菌株 no
Comments 注釋 Nutritional study of Naegleria gruberi
Medium 培養(yǎng)基 ATCC? Medium 1034: Modified PYNFH medium (Available from ATCC as ATCC cat. no. 327-X)
ATCC? Medium 710: Nelson's Culture Medium For Naegleria
ATCC? Medium 902: Schuster's axenic Naegleria medium
Growth Conditions 生長(zhǎng)條件
Temperature 培養(yǎng)溫度: 25°C
Culture System: Axenic
Harvest and Preservation 1，Harvest cells from a culture that is at or near peak density by centrifugation at 600 x g for 5 min. Pool the cell pellets into a single tube.
2，Adjust the concentration of cells to 2.0 x 106/mL. If the concentration is too low, centrifuge at 600 x g for 5 minutes and resuspend the cell pellet with a volume of supernatant to yield the desired concentration.
3，Prepare a 15% (v/v) sterile DMSO solution in ATCC medium 1034 as follows: Add the required volume of DMSO to a glass screw-capped test tube and place on ice. Allow the DMSO to solidify. Add the required volume of refrigerated ATCC medium 1034. Dissolve the DMSO by inverting several times. If the DMSO solution is not prepared on ice, an exothermic reaction will occur that may precipitate certain components of the medium.
4，Mix the cell preparation and the DMSO in equal portions. Thus, the final concentration will be 106 and 7.5% (v/v) DMSO. The time from the mixing of the cell preparation and DMSO stock solution to the start of the freezing process should be no less than 15 min and no longer than 60 min.
5，Dispense in 0.5 mL aliquots into 1.0 - 2.0 mL sterile plastic screw-capped cryules (special plastic vials for cryopreservation).
6，Place vials in a controlled rate freezing unit. From room temperature cool at -1°C/min to -40°C. If freezing unit can compensate for the heat of fusion, maintain rate at -1°C/min through heat of fusion. At -40°C plunge ampules into liquid nitrogen.
7，The frozen preparations are stored in either the vapor or liquid phase of a nitrogen refrigerator.
8，To establish a culture from the frozen state place an ampule in a water bath set at 35°C. Immerse the vial just sufficiently to cover only the frozen material. Do not agitate the vial.
9，Immediately after thawing, do not leave in the water bath, aseptically remove the contents of the ampule and inoculate into a fresh tube or flask of ATCC medium 1034. 10，Incubate at 25°C with the cap screwed on tightly (incubate a test tube on a 15° horizontal slant).Name of Depositor WD ODell Year of Origin 1970
References O'Dell WD, Brent MM. Nutritional study of three strains of Naegleria gruberi. J. Protozool. 21: 129-133, 1974. PubMed: 4206403
O'Dell WD, Stevens AR. Quantitative growth of Naegleria in axenic culture. Appl. Microbiol. 25: 621-627, 1973. PubMed: 4633479
De Jonckheere JF. A century of research on the amoeboflagellate genus Naegleria. Acta Protozool. 41: 309-342, 2002.
