ATCC? Number:30572?
Organism: Crypthecodinium cohnii (Seligo) Javornicky
Designations: PS-1
Isolation: Fucus sp., Puget Sound, WA, 1976
Depositors: CA Beam, M Himes
Biosafety Level:1
Shipped: frozen
Growth Conditions: ATCCmedium 460: A2E6 mediumTemperature: 25.0°C Duration: axenic Protocol: ATCCNO: 30021 SPEC: This culture is routinely shipped as a frozen stabilate. Thaw the ampule and aseptically transfer the material to 5 ml of ATCCmedium 460 in a 16 x 125 mm screw-capped test tube. Do not distribute the thawed material to a larger volume of medium. It is essential to first establish the culture in a small volume. Screw cap on tightly, loosen one half turn, and incubate culture upright at 25C. The culture should be ready to subculture in approximately 7 days. To subculture, screw the cap on tightly, invert the culture 5 times and aseptically transfer a 0.1 ml aliquot to 5 ml of ATCCmedium 460 and incubate as above. Prepare two subcultures weekly. Retain all cultures for up to one month to ensure against loss.
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Applications: produces docosahexaenoic acids docosahexaenoic acid [5190] [4571]
Comments: Major Sibling Species WH sexuality and meiosis [21756] genetic diversification [4790]
Classification: KINGDOM: Protozoa
References: 4571: . . Japanese Patent 7,008,221 4790: Beam CA, Himes M. Sexual isolation and genetic diversification among some strains of Crypthecodinium cohnii-like dinoflagellates evidence of speciation. J. Protozool. 24: 532-539, 1977. 5124: Seafood products having good taste containing marine microalgae, particularly Crythecodinium cohnii containing docosahexaenoic acid. Japanese Patent 7,008,221 5190: Iizuka T, et al. Marine micro-algae food material containing docosahexaenoic acid, food containing the same and manufacturing method therefor. US Patent 5,547,699 dated Aug 20 1996 21756: Beam CA, Himes MSexuality and meiosis in dinoflagellatesIn: Beam CA, Himes MBiochemistry and physiology of protozoa, 2nd ed.3New YorkAcademic Press171-206, 1980 23853: Beam CA, et al. Genetic evidence of unusual meiosis in the dinoflagellate Crypthecodinium cohnii. Genetics 87: 19-32, 1977.