ATCC Number:30544
Organism: Naegleria clarki De Jonckheere
Designations: PD
Isolation: sewage effluent, Ohio, 1969
Depositors: WD ODell
Biosafety Level:1
Shipped: dried
Growth Conditions: ATCCmedium 997: Fresh water ameba mediumTemperature: 25.0°C Duration: grown with Escherichia coli ATCC11775 Protocol: ATCCNO: 30133 SPEC: This strain is shipped as a dried preparation. Aseptically add 1 ml of sterile distilled water to the inner vial, remove the filter paper pellet with a pair of forceps, and place it in the center of an agar plate of ATCCmedium 711 or 997. Add the liquid remaining in the vial to the plate and spread it evenly over the surface of the plate. Incubate the plate at 25C. Trophozoites (amebae) should be evident within 2-3 days.
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Comments: Maximum temperature tolerance 37C [23932] Maximum temperature tolerance 39C [38900] This strain and ATCC50357 are members of Naegleria sp. genetic cluster G [38900] Nutritional study of Naegleria gruberi [4776] genetic cluster G [38900]
References: 4776: O'Dell WD, Brent MM. Nutritional study of three strains of Naegleria gruberi. J. Protozool. 21: 129-133, 1974. PubMed: 4206403 23932: Adams M, et al. A genetic approach to species criteria in the amoeba genus Naegleria using allozyme electrophoresis. Int. J. Parasitol. 19: 823-834, 1989. PubMed: 2635158 38900: Robinson BS, et al. Discontinuous genetic variation among mesophilic Naegleria isolates: Further evidence that N. gruberi is not a single species. J. Protozool. 39: 702-712, 1992. PubMed: 1453360 61465: De Jonckheere JF. A century of research on the amoeboflagellate genus Naegleria. Acta Protozool. 41: 309-342, 2002. 61469: De Jonckheere JF. Evidence for the ancestral origin of group I introns in the SSUrDNA of Naegleria spp.. J. Eukaryot. Microbiol. 41: 457-463, 1994. PubMed: 7804245