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平臺編號：bio-48384
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國際編號：ATCC 30487
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拉丁屬名： Acanthamoeba polyphaga
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規(guī)格：dried
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用途：ATCC原裝進(jìn)口
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服務(wù)費用：
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注意事項：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準(zhǔn)）
多食棘阿米巴
ATCC? Number:30487?
Organism: Acanthamoeba polyphaga (Puschkarew) Page
Designations: Panola Mountain
Isolation: soil, Panola Mountain, DeKalb County, GA, 1975
Depositors: WS Jenkins
Biosafety Level:1
Shipped: dried
Growth Conditions: ATCCmedium 711: PYBTemperature: 25.0°C Duration: grown with bacteria Protocol: ATCCNO: 30011 SPEC: This strain is distributed as a dried preparation. See the general procedures for opening a dried vial. Aseptically add 1 ml of sterile distilled water to the inner shell vial, remove the filter paper aseptically with a pair of forceps, and place it in the center of an agar plate of ATCCmedium 997. Add the liquid remaining in the vial to the plate and spread it evenly over the surface of the plate. Incubate the plate at 25C. Trophozoites (amebae) should be evident within 2-3 days.
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Comments: Indistinguishable from Acanthamoeba polyphaga ATCC30486 and very similar to A. griffini ATCC30731 on the basis of starch gel electrophoretic analysis of enzymes. phylogeny [24028]
Cross References: Nucleotide (GenBank) : AF019052 Acanthamoeba polyphaga Panola Mountain 18S ribosomal RNA gene, partial sequence.
Classification: KINGDOM: Protozoa
References: 4594: Jenkins WS. The role of the contractile vacuole in excystment of Acanthamoeba polyphaga (Puschkarew) Page. J. Protozool. 23: 24A, 1976. 23896: Daggett PM, et al. Distribution and possible interrelationships of pathogenic and nonpathogenic Acanthamoeba from aquatic environments. Microb. Ecol. 8: 371-386, 1982. 24028: Daggett PM, et al. A molecular approach to the phylogeny of Acanthamoeba. Biosystems 18: 399-405, 1985. PubMed: 4084681 32162: Stothard DR, et al. The evolutionary history of the genus Acanthamoeba and the identification of eight new 18S rRNA gene sequence types. J. Eukaryot. Microbiol. 45: 45-54, 1998. PubMed: 9495032 43732: Stothard DR, et al. Fluorescent oligonucleotide probes for clinical and environmental detection of Acanthamoeba and the T4 18S rRNA gene sequence type. J. Clin. Microbiol. 37: 2687-2693, 1999. PubMed: 10405422 70805: Ledee DR, et al. Advantages of using mitochondrial 16S rDNA sequences to classify clinical isolates of Acanthamoeba. Invest. Ophthalmol. Vis. Sci. 44: 1142-1149, 2003. PubMed: 12601042
