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平臺(tái)編號(hào)：bio-48404
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國際編號(hào)：ATCC 30467
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拉丁屬名： Naegleria lovaniensis
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規(guī)格：frozen
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用途：ATCC原裝進(jìn)口
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服務(wù)費(fèi)用：
加載中…… - 訂購
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注意事項(xiàng)：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動(dòng)物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準(zhǔn)）
K-1
Naegleria lovaniensis Steven et al.拉丁名
(ATCC? 30467?) 統(tǒng)一編號(hào)
Deposited As Naegleria fowleri Carter
Strain Designations 菌株別名 K-1
Biosafety Level 生物安全等級(jí) 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Isolation domestic water supply, Kadina, Australia, 1972
Product Format 提供形式 frozen
Type Strain 模式菌株 no
Comments 注釋 received axenic, distributed monoxenic with Escherichia coli ATCC 11775
Medium 培養(yǎng)基 ATCC? Medium 919: Non-nutrient agar
Growth Conditions 生長條件
Temperature 培養(yǎng)溫度: 35.0°C
Duration: grown with Escherichia coli ATCC 11775
Cryopreservation 1. Allow the cells to encyst. To detach cysts from the plate flush the surface with 5 ml fresh ATCC medium 1323 (Page's Balanced Salt Solution). Rub the surface of the plate with a spread bar to detach adhering amoebae.
2. Transfer the cyst suspension to a sterile centrifuge tube.
3. If the cyst concentration does not exceed 2 x 106 cysts/ml adjust the suspension to that concentration. To adjust the concentration, centrifuge at 600 x g for 5 min and resuspend the pellet in the volume of fresh medium required to yield 2 x 106.
4. While cells are centrifuging prepare a 15% (v/v) solution of sterile DMSO as follows: Add the required volume of DMSO to a glass screw-capped test tube and place it in an ice bath. Allow the DMSO to solidify. Add the required volume of refrigerated medium. Dissolve the DMSO by inverting the tube several times. Name of Depositor JA Jamieson
Year of Origin 收藏年份 1972
References 參考文獻(xiàn) Anderson K, Jamieson A. Bacterial suspensions for the growth of Naegleria species. Pathology 6: 79-84, 1974. PubMed: 4207323
De Jonckheere JF. A century of research on the amoeboflagellate genus Naegleria. Acta Protozool. 41: 309-342, 2002.
