亚日韩在线中文字幕亚洲,国产在线播放鲁啊鲁视频,张柏芝手扒性器全部图片,亚洲成无码电影在线观看

Ulothrix gigas (Vischer) Mattox and Bold 拉丁名

(ATCC? 30443?) 統(tǒng)一編號(hào)

Deposited As Uronema gigas Vischer 

Strain Designations 菌株別名 UTEX 174 [CCAP-386/3] 

Application 用途 algal resistance testing paint 

Biosafety Level 生物安全等級(jí) 1 

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. 

Isolation 分離基物 Freshwater, Switzerland, 1930 

Product Format frozen 

Storage Conditions 保藏條件

Frozen Cultures: -70°C for 1 week; liquid N2 vapor for long term storage 

Freeze-dried Cultures: 2-8°C 

Live Cultures: See Protocols section for handling information 

Type Strain 模式菌株 no 

Comments 注釋 This strain is recommended by ATCC for use in the tests described in ASTM Standard Test Method E1366-91 where only the taxon is specified. photosynthetic

Medium 培養(yǎng)基 ATCC? Medium 847: Algal proteose agar

Growth Conditions 生長(zhǎng)條件

Temperature 培養(yǎng)溫度: 25°C 

Culture System: Axenic 

Cryopreservation Harvest and Preservation

1，Harvest cells from a culture that is at or near peak density by centrifugation at 800 x g for 5 min.,

2，Adjust the concentration of cells to 2 x 106 - 2 x 107/mL in fresh broth medium. ,

3， cells are centrifuging prepare a 14% (v/v) solution of sterile DMSO in fresh broth medium. ,

4， the cell preparation and the 14% DMSO in equal portions. Thus, the final concentration will be 106 - 107 cells/mL and 7% (v/v) DMSO. The time from the mixing of the cell preparation and DMSO stock solution to the beginning of the freezing process should be no less than 5 min and no greater than 15 min. 

5，Dispense in 0.5 mL aliquots into 1.0 - 2.0 mL sterile plastic screw-capped cryules (special plastic vials for cryopreservation)., 

6，Place the vials in a controlled rate freezing unit.  From room temperature cool at -1°C/min to -40°C.  If the freezing unit can compensate for the heat of fusion, maintain rate at -1°C/min through the heat of fusion.  At -40°C plunge into liquid nitrogen. Alternatively, place the vials in a Nalgene 1°C freezing apparatus.  Place the apparatus at -80°C for 1.5 to 2 hours and then plunge ampules into liquid nitrogen.  (The cooling rate in this apparatus is approximately -1°C/min.)  

7，The frozen preparations should be stored in either the vapor or liquid phase of a nitrogen refrigerator. Frozen preparations stored below -130°C are stabile indefinitely. Those stored at temperatures above -130°C are progressively less stabile as the storage temperature is elevated. Vials should not be stored above -55°C. 

8，To establish a culture from the frozen state place an ampule in a water bath set at 35°C. Immerse the vial just to a level just above the surface of the frozen material. Do not agitate the vial.,

9，Immediately after thawing, do not leave in the water bath, aseptically remove the contents of the ampule and inoculate a 16 x 125 mm screw-capped test tube containing 5 mL of ATCC medium 847 broth.,

10，Incubate the culture on a 15° horizontal slant at 25°C with the cap screwed on loosely (loosened one-half turn) and incubate under a 14 hour light (~50 μEinsteins/m2/s irradiance)/10 hour dark cycle.

Name of Depositor UTEX 

Chain of Custody ATCC <-- UTEX <-- . . . <-- W. Vischer strain 69 

Year of Origin 收藏年份 1930 

References 參考文獻(xiàn) Starr RC. The culture collection of algae at Indiana University. Am. J. Bot. 51: 1013-1044, 1964. Standard Test Method for Determining the Resistance of Paint Films and Related Coatings to Algal Defacement. West Conshohocken, PA:ASTM International;ASTM Standard Test Method D 5589-97 (Reapproved 2002).