ATCC? Number:30399?
Organism: Didinium nasutum (Muller) Stein
Isolation: freshwater pond, San Francisco, CA, 1958
Depositors: W Balamuth
Biosafety Level:1
Shipped: test tube
Growth Conditions: ATCCmedium 802: Sonneborn's Paramecium mediumTemperature: 25.0°C Protocol: ATCCNO: 30399 SPEC: This strain is distributed as a dried preparation. See the general procedures for opening a dried vial. Aseptically, add 1 ml of ATCCmedium 802 inoculated 24 hours previously with bacteria (e.g. Enterobacter aerogenes ATCC-13048) to the inner shell vial. Once completely rehydrated, aseptically transfer the material to a T-25 tissue culture flask containing a thriving culture of Paramecium, not provided. Place the flask at at 25C. Excystment should occur within a few days.
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Comments: Changes in the cell volume [4783] Preservation [5007]
Classification: KINGDOM: Protozoa
References: 4783: Salt GW. Changes in the cell volume of Didinium nasutum during population increase. J. Protozool. 22: 112-115, 1975. 5007: McGrath MS, et al. Studies on the preservation of the ciliate Didinium nasutum. Trans. Am. Microsc. Soc. 96: 519-525, 1977.