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平臺編號：bio-48523
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國際編號：ATCC 30297
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拉丁屬名： Tokophrya infusionum
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規(guī)格：test tube
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用途：ATCC原裝進(jìn)口
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注意事項(xiàng)：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準(zhǔn)）
clone 25
ATCC? Number:30297?
Organism: Tokophrya infusionum (Stein) Collin
Designations: clone 25
Isolation: pool at Rockefeller University, New York City, 1959
Depositors: MA Rudzinska
Biosafety Level:1
Shipped: test tube
Growth Conditions: ATCCmedium 875: AUYMax Temperature: 25.0°C Min Temperature: 19.0°C Duration: grown with Tetrahymena borealis ATCC30321 Protocol: ATCCNO: 30297 SPEC: Tokophrya infusionum must be periodically fed Tetrahymena (e.g., T. borealis ATCC30321). Two methods may be used: 1) Prior to subculturing, feed 0.03 ml of a 5- to 7-day-old culture of ATCC30321 to T. infusionum. A large number of embryos will be formed 1-4 hours after feeding. Transfer 1 ml of medium containing the free-swimming embryos to new medium. The number of embryos is variable depending upon the state of the culture and the number of tetrahymenas fed. 2) If difficulty is encountered in using method 1, the following method can be used with satisfactory results: Place the stock culture in an ice bath for 15-20 min., then agitate it. Some adults will detach from the glass surface. Transfer 1.0-2.0 ml of the medium containing dislodged adults to fresh medium and immediately feed with 0.03 ml of Tetrahymena. Four to five days after subculturing, feed the cultures again with 0.3 ml of Tetrahymena. Cultures are usually maintained in 20 X 125-mm screw-capped tubes containing 15.0 ml, kept upright with caps loosened. Cells attach in the region of the meniscus.
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Comments: Localization of acid phosphatase [4781] use of plastic ampoules for freeze preservation [23870]
Classification: KINGDOM: Protozoa
References: 4781: Rudzinska MA. Ultrastructural localization of acid phosphatase in starved Tokophrya infusionum. J. Protozool. 21: 721-728, 1974. PubMed: 4217373 23870: Simione FP Jr., et al. The use of plastic ampoules for freeze preservation of microorganisms. Cryobiology 14: 500-502, 1977. PubMed: 891238
