ATCC® Number:30298™
Organism: Vahlkampfia lobospinosa (Craig) Craig
Isolation: cattle feces, Shelby County, TN, 1969
Depositors: WE Wilhelm, ER Fizer
Biosafety Level:1
Shipped: dried
Growth Conditions: ATCCmedium 711: PYBTemperature: 35.0°C Duration: grown with Escherichia coli ATCC11775 Protocol: ATCCNO: 30133 SPEC: This strain is shipped as a dried preparation. Aseptically add 1 ml of sterile distilled water to the inner vial, remove the filter paper pellet with a pair of forceps, and place it in the center of an agar plate of ATCCmedium 711 or 997. Add the liquid remaining in the vial to the plate and spread it evenly over the surface of the plate. Incubate the plate at 25C. Trophozoites (amebae) should be evident within 2-3 days.
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Comments: Circular ribosomal RNA genes [4814] biochemical identification [4799] Restriction endonuclease analysis of mitochondrial DNA [4813] Identification and phylogenetic relationships [23863] Promitosis [23992] use of plastic ampoules for freeze preservation [23870]
Cross References: Nucleotide (GenBank) : M98052 Vahlkampfia lobospinosa 16S ribosoma RNA-like gene.
References: 4799: Daggett PM, Nerad TA. The biochemical identification of vahlkampfiid amoebae. J. Protozool. 30: 126-128, 1983. PubMed: 6864593 4813: Milligan SM, Band RN. Restriction endonuclease analysis of mitochondrial DNA as an aid in the taxonomy of Naegleria and Vahlkampfia. J. Protozool. 35: 198-204, 1988. PubMed: 2840491 4814: Clark CG, Cross GA. Circular ribosomal RNA genes are a general feature of schizopyrenid amoebae. J. Protozool. 35: 326-329, 1988. PubMed: 2840492 5056: Wilhelm WE, Anderson JH. Vahlkampfia lobospinosa (Craig. 1912) Craig. 1913: rediscovery of a coprozoic ameba. J. Parasitol. 57: 1378-1379, 1971. PubMed: 5157177 23863: Brown S, De Jonckheere JF. Identification and phylogenetic relationships of Vahlkampfia spp. (free-living amoebae) by riboprinting. FEMS Microbiol. Lett. 115: 241-246, 1994. 23870: Simione FP Jr., et al. The use of plastic ampoules for freeze preservation of microorganisms. Cryobiology 14: 500-502, 1977. PubMed: 891238 23992: Fizer ER, Wilhelm WE. Promitosis and rod occurrence in the limax ameba Vahlkampfia lobospinosa. J. Protozool. 25: 34-38, 1978. PubMed: 660570
