ATCC? Number:30294?
Organism: Naegleria galeacystis De Jonckheere
Designations: A.V. 500
Isolation: soil, Garden City, NY, 1967
Depositors: JJ Napolitano
Biosafety Level:1
Shipped: dried
Growth Conditions: ATCCmedium 839: 12YAATCCmedium 997: Fresh water ameba mediumTemperature: 25.0°C Duration: grown with Enterobacter aerogenes ATCC13048 Protocol: ATCCNO: 30294 SPEC: This strain is shipped as a dried preparation. Aseptically add 1 ml of sterile distilled water to the inner vial, remove the filter paper pellet with a pair of forceps, and place it in the center of an agar plate of ATCCmedium 711 or 997. Add the liquid remaining in the vial to the plate and spread it evenly over the surface of the plate. Incubate at 25C. Trophozoites (amebae) should be evident within 2-3 days.
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments: Original description of Adelphamoeba galeacystis - The strain deposited was not the same as that described species description [4766] Circular ribosomal RNA genes [4814] Transfer to genus Naegleria based on SSU rRNA gene phylogeny [21639] Biochemical identification [4799]
References: 4766: Napolitano JJ, et al. Adelphamoeba galeacystis n. g., n. sp., an amoeboflagellate isolated from soil. J. Protozool. 17: 158-161, 1970. 4799: Daggett PM, Nerad TA. The biochemical identification of vahlkampfiid amoebae. J. Protozool. 30: 126-128, 1983. PubMed: 6864593 4814: Clark CG, Cross GA. Circular ribosomal RNA genes are a general feature of schizopyrenid amoebae. J. Protozool. 35: 326-329, 1988. PubMed: 2840492 21639: De Jonckheere JF, et al. On the identity of the amoeboflagellates Didascalus thorntoni and Adelphamoeba galeacystis. J. Eukaryot. Microbiol. 44: 52-54, 1997. PubMed: 9172832 23932: Adams M, et al. A genetic approach to species criteria in the amoeba genus Naegleria using allozyme electrophoresis. Int. J. Parasitol. 19: 823-834, 1989. PubMed: 2635158 38899: De Jonckheere JF. Comparison of partial SSU rRNA sequences suggests revisions of species names in the genus Naegleria. Eur. J. Protistol. 30: 333-341, 1994.