ATCC Number:30264
Organism:
Crithidia oncopelti (Noguchi and Tilden) Hanson and McGhee
Isolation: Aposymbiotic
Depositors: KP Chang
History: ATCC<<--KP Chang<<--ATCC12982
Biosafety Level:1
Shipped: frozen
Growth Conditions: ATCCmedium 355: Crithidia medium
Temperature: 25.0°C
Duration: axenic Protocol: ATCCNO: 11745 SPEC: See general instructions for thawing and storage of frozen material before proceeding. Add thawed contents to a single 16 x 125 mm glass screw-capped test tube of the appropriate medium. Incubate the culture vertically with the cap screwed on tightly. It is essential to establish cultures initially in small volumes. Once established, the culture can be scaled up to larger volumes. Vigorously agitate the culture and aseptically transfer 0.1 ml of culture to a fresh tube of medium weekly.
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Comments: species description [4825] Ornithine-arginine metabolism [4786] Ultrastructure of symbiotic bacteria [4780] Proteolytic activities [23776] isoleucine requirement and threonine deaminase [24118] endonuclease-generated fragments of K-DNA, esterase isoenzymes, surface proteins for species identification [24216] Acetylornithinase and ornithine acetyltransferase [24012] Ultrastructural differences between species with and without endosymbionts [24077]
Cross References: Nucleotide (GenBank) : U67436 Crithidia oncopelti large subunit 24S rRNA gene, 5'end, LS1 region.
References: 4780: Chang KP. Ultrastructure of symbiotic bacteria in normal and antibiotic-treated Blastocrithidia culicis and Crithidia oncopelti. J. Protozool. 21: 699-707, 1974. PubMed: 4217371 4786: Figueiredo EN, et al. Enzymes of the ornithine-arginine metabolism of trypanosomatids of the genus Crithidia. J. Protozool. 25: 546-549, 1978. 4792: Chang KP, et al. Effects of methylglyoxal bis(ganylhydrazone) on trypanosomatid flagellates: inhibition of growth and nucleoside incorporation in Trypanosoma brucei. J. Protozool. 25: 145-149, 1978. PubMed: 660567 4825: Faria e Silva PM, et al. Herpetomonas roitmani (Fiorini et al., 1989) n. comb.: a trypanosomatid with a bacterium-like endosymbiont in the cytoplasm. J. Protozool. 38: 489-494, 1991. PubMed: 1920148 23776: Camargo EP, et al. Proteolytic activities in cell extracts of trypanosomatids. J. Parasitol. 64: 1120-1121, 1978. PubMed: 739304 24012: Galinari S, Camargo EP. Trypanosomatid protozoa: survey of acetylornithinase and ornithine acetyltransferase. Exp. Parasitol. 46: 277-282, 1978. PubMed: 569594 24077: Freymuller E, Camargo EP. Ultrastructural differences between species of trypanosomatids with and without endosymbionts. J. Protozool. 28: 175-182, 1981. PubMed: 7024533 24118: Alfieri SC, Camargo EP. Trypanosomatidae: isoleucine requirement and threonine deaminase in species with and without endosymbionts. Exp. Parasitol. 53: 371-380, 1982. PubMed: 6806116 24216: Camargo EP, et al. Electrophoretic analysis of endonuclease-generated fragments of k-DNA, of esterase isoenzymes, and of surface proteins as aids for species identification of insect trypanosomatids. J. Protozool. 29: 251-258, 1982. PubMed: 6284925