ATCC® Number:30266™
Organism: Trypanosoma cruzi Chagas
Designations: Tulahuen
Isolation: Triatoma infestans, Tulahuen, Chile, 1945
Depositors: RG Yaeger
History: ATCC<<--RG Yaeger<<--T. Pizzi
Biosafety Level:2
Shipped: frozen
Growth Conditions: ATCCmedium 1011: Diphasic blood agar mediumTemperature: 25.0°C Growth condition: axenic Protocol: See general instructions for thawing and storage of frozen material before proceeding. Add thawed contents to a single 16 x 125 mm glass screw-capped test tube of the appropriate medium. Incubate the culture vertically with the cap screwed on tightly. It is essential to establish cultures initially in small volumes. Once established, the culture can be scaled up to larger volumes. Vigorously agitate the culture and aseptically transfer 0.1 ml of culture to a fresh tube of medium weekly.
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Comments: antigenic differentiation [24169]
Classification: KINGDOM: Protozoa
References: 4713: . . Exp. Parasitol. 9: 215-222, 1960. 5174: Immunologia de la Enfermedad de Chagas. Santiago, Chile: Universidad de Chile; 24169: Constantine NT, Anthony RL. Antigenic differentiation of the kinetoplastids Leishmania braziliensis and Trypanosoma cruzi by means of monoclonal antibodies. J. Protozool. 30: 346-350, 1983. PubMed: 6195336
