ATCC? Number:30268?
Organism: Blastocrithidia culicis (Novy et al.) Wallace and Johnson
Isolation: mosquito, Aedes vexans, 1942
Depositors: KP Chang
History: ATCC<<--KP Chang<<--F.G. Wallace
Biosafety Level:1
Shipped: frozen
Growth Conditions: ATCCmedium 832: Grace?s insect mediumTemperature: 25.0°C Duration: axenic Protocol: ATCCNO: 11745 SPEC: See general instructions for thawing and storage of frozen material before proceeding. Add thawed contents to a single 16 x 125 mm glass screw-capped test tube of the appropriate medium. Incubate the culture vertically with the cap screwed on tightly. It is essential to establish cultures initially in small volumes. Once established, the culture can be scaled up to larger volumes. Vigorously agitate the culture and aseptically transfer 0.1 ml of culture to a fresh tube of medium weekly.
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Comments: Endosymbiont-bearing Nutrition significance of symbiotic bacteria [4906] monophyly of endosymbiont-containing trypanosomatids [38603] Effects of methylglyoxal bis (guanylhydrazone) on growth [4792] Ultrastructure of symbiotic bacteria [4780] RNA editing [23998] Cell-to-cell interactions [24090] Trypomastigotes in cultures [24122] Cyclopropane fatty acid [24043]
Cross References: Nucleotide (GenBank) : L29266 ribosomal RNA sequenceNucleotide (GenBank) : U05814 kinetoplast NADH dehydrogenase subunit 7 (ND7) gene, partial coding sequence, and cytochrome c oxidase subunit III (COIII) gene, complete coding sequenceNucleotide (GenBank) : U05817 kinetoplast NADH dehydrogenase subunit 7 (ND7) gene, partial pre-RNA-edited DNA sequenceNucleotide (GenBank) : L29265 Blastocrithidia culicis ribosomal RNA.Nucleotide (GenBank) : U05813 kinetoplast (MURF1) and apocytochrome B (cytB) genes, partial coding sequence, and ATPase subunit 6 (MURF4) gene, complete pre-RNA-edited DNA sequence
References: 4780: Chang KP. Ultrastructure of symbiotic bacteria in normal and antibiotic-treated Blastocrithidia culicis and Crithidia oncopelti. J. Protozool. 21: 699-707, 1974. PubMed: 4217371 4792: Chang KP, et al. Effects of methylglyoxal bis(ganylhydrazone) on trypanosomatid flagellates: inhibition of growth and nucleoside incorporation in Trypanosoma brucei. J. Protozool. 25: 145-149, 1978. PubMed: 660567 4906: Chang KP, Trager W. Nutritional significance of symbiotic bacteria in two species of hemoflagellates. Science 183: 531-532, 1974. PubMed: 4203488 23998: Maslov DA, et al. Evolution of RNA editing in kinetoplastid protozoa: pan-editing preceded 5'-editing. Nature 368: 345-348, 1994. PubMed: 8127370 24043: Fish WR, et al. The cyclopropane fatty acid of trypanosomatids. Mol. Biochem. Parasitol. 3: 103-115, 1981. PubMed: 7254247 24090: Sousa MA. Cell-to-cell interactions suggesting a sexual process in Herpetomonas megaseliae (Kinetoplastida: Trypanosomatidae). Parasitol. Res. 80: 112-116, 1994. PubMed: 8202449 24122: de Sousa MA. Trypomastigotes in cultures of Blastocrithidia culicis (Novy, MacNeal & Torrey, 1907) (Kinetoplastida: Trypanosomatidae). Mem. Inst. Oswaldo Cruz Rio J. 89: 111-112, 1994. PubMed: 7823806 38603: Hollar J, et al. Monophyly of endosymbiont containing trypanosomatids: Phylogeny versus taxonomy. J. Eukaryot. Microbiol. 45: 293-297, 1998. PubMed: 9627990 38755: Du Y, et al. Monophyletic origin of beta-division proteobacterial endosymbionts and their coevolution with insect trypanosomatid protozoa. Proc. Natl. Acad. Sci. USA 91: 8437-8441, 1994. PubMed: 7521530