ATCC? Number:30287?
Organism: Phytomonas davidi Lafont
Isolation: Euphorbia heterophylla, Ocochobee City, FL, 1975
Depositors: RB McGhee 
Biosafety Level:1
Shipped: frozen 
Growth Conditions: ATCCmedium 355: Crithidia mediumTemperature: 25.0°C Duration: axenic Protocol: ATCCNO: 11745 SPEC: See general instructions for thawing and storage of frozen material before proceeding. Add thawed contents to a single 16 x 125 mm glass screw-capped test tube of the appropriate medium. Incubate the culture vertically with the cap screwed on tightly. It is essential to establish cultures initially in small volumes. Once established, the culture can be scaled up to larger volumes. Vigorously agitate the culture and aseptically transfer 0.1 ml of culture to a fresh tube of medium weekly.
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Comments: Transmission and ultrastructure  [23781] conservation of a 29-base-pair sequence within maxicircle ARS fragments  [24079] Unique no-branched polyunsaturated fatty acids  [24089] Cyclopropane fatty acid  [24043] isoleucine requirement and threonine deaminase  [24118]
References:  4592: . .  J. Protozool. 22: 10A, 1975. 23781: Postell FJ, McGhee RB. Phytomonas davidi (Trypanosomatidae): transmission and ultrastructure.  J. Protozool. 22: 10A, abstract 9, 1975. 24043: Fish WR, et al. The cyclopropane fatty acid of trypanosomatids.  Mol. Biochem. Parasitol. 3: 103-115, 1981. PubMed: 7254247 24079: Kim R, Ray DS. Conservation of a 29-base-pair sequence within maxicircle ARS fragments from six species of trypanosomes.  Gene 40: 291-299, 1985. PubMed: 3938423 24089: Fish WR, et al. Some Phytomonas and Herpetomonas species form unique iso-branched polyunsaturated fatty acids.  Mol. Biochem. Parasitol. 5: 1-18, 1982. PubMed: 7062937 24118: Alfieri SC, Camargo EP. Trypanosomatidae: isoleucine requirement and threonine deaminase in species with and without endosymbionts.  Exp. Parasitol. 53: 371-380, 1982. PubMed: 6806116 34222: Cho J, Eichinger D. Crithidia fasciculata induces encystation of Entamoeba invadens in a galactose-dependent manner.  J. Parasitol. 84: 705-710, 1998. PubMed: 9714198