ATCC? Number:30282?
Organism: Trypanosoma cyclops Weinman
Designations: 7549
Isolation: monkey, Macaca nemestrina, West Malaysia, 1969
Depositors: D Weinman
Biosafety Level:2
Shipped: frozen
Growth Conditions: ATCCmedium 1011: Diphasic blood agar mediumTemperature: 25.0°C Duration: axenic Protocol: ATCCNO: 11745 SPEC: See general instructions for thawing and storage of frozen material before proceeding. Add thawed contents to a single 16 x 125 mm glass screw-capped test tube of the appropriate medium. Incubate the culture vertically with the cap screwed on tightly. It is essential to establish cultures initially in small volumes. Once established, the culture can be scaled up to larger volumes. Vigorously agitate the culture and aseptically transfer 0.1 ml of culture to a fresh tube of medium weekly.
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Type Strain: yes(type strain)
Comments: Cloned strain. Variation in kinetoplast minicircle and maxicircle DNA sequences [24219]
Classification: KINGDOM: Protozoa
References: 4918: Weinman D. Trypanosoma cyclops n. sp.: a pigmented trypanosome from the Malaysian primates Macaca nemestrina and M. ira. Trans. R. Soc. Trop. Med. Hyg. 66: 628-636, 1972. PubMed: 4627177 24219: Gomez-Eichelmann MC, et al. Comparison of several lizard Leishmania species and strains in terms of kinetoplast minicircle and maxicircle DNA sequences, nuclear chromosomes, and membrane lipids [published erratum appears in Mol. Biochem. Parasitol. 28: 301, 1988]. Mol. Biochem. Parasitol. 27: 143-158, 1988. PubMed: 3344003