ATCC® Number:30267™
Organism: Leishmania tarentolae Parrot
Designations: Tar II
Isolation: lizard, Tarentola mauritanica, Biakra, Algeria, 1934
Depositors: W Trager
History: ATCC<<--W Trager<<--L. Parrot
Biosafety Level:1
Shipped: frozen
Growth Conditions: ATCCmedium 1011: Diphasic blood agar mediumTemperature: 25.0°C Duration: axenic Protocol: ATCCNO: 11745 SPEC: See general instructions for thawing and storage of frozen material before proceeding. Add thawed contents to a single 16 x 125 mm glass screw-capped test tube of the appropriate medium. Incubate the culture vertically with the cap screwed on tightly. It is essential to establish cultures initially in small volumes. Once established, the culture can be scaled up to larger volumes. Vigorously agitate the culture and aseptically transfer 0.1 ml of culture to a fresh tube of medium weekly.
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Comments: Promastigotes. Methods for screening compounds [23854] Cysteine and metalloproteinase activities [23887]
References: 4688: . . J. Protozool. 4: 269-276, 1957. 23854: Stauber LA, et al. An eight-day method for screening compounds against Leishmania donovani in the golden hamster. J. Protozool. 5: 269-273, 1958. 23887: Branquinha MH, et al. Ubiquity of cysteine-and metalloproteinase activites in a wide range of trypanosomatids. J. Eukaryot. Microbiol. 43: 131-135, 1996. PubMed: 8720943
