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Leptomonas collosoma Wallace et al. 拉丁名

(ATCC? 30261?) 統(tǒng)一編號(hào)

Biosafety Level 生物安全等級(jí) 1 

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. Isolation Gerris dissortis, Minneapolis, MN, 1960 

Product Format 提供形式 frozen 

Type Strain 模式菌株 yes 

Comments 注釋 Trypanosomatids from fruit; 

Distribution of carbohydrate epitopes; 

Two new species from genus Gerris; 

presences in feces of lizards fed culture forms of the flagellates; 

RNA editing; 

Proteolytic activities; 

enzymes of ornithine-arginine metabolism; 

Monoclonal antibodies for identification; 

Acetylornithinase and ornithine acetyltransferase; 

Ultrastructural differences between species with and without endosymbionts; 

Cyclopropane fatty acid; 

Medium 培養(yǎng)基 ATCC? Medium 1011: Diphasic blood agar medium 

Growth Conditions 生長(zhǎng)條件

Temperature 培養(yǎng)溫度: 25.0°C 

Duration: axenic 

Protocol: ATCC NO: 11745 SPEC: See general instructions for thawing and storage of frozen material before proceeding. Add thawed contents to a single 16 x 125 mm glass screw-capped test tube of the appropriate medium. Incubate the culture vertically with the cap screwed on tightly. It is essential to establish cultures initially in small volumes. Once established, the culture can be scaled up to larger volumes. Vigorously agitate the culture and aseptically transfer 0.1 ml of culture to a fresh tube of medium weekly. 

Subcultivation Protocol: ATCC NO: 11745 SPEC: See general instructions for thawing and storage of frozen material before proceeding. Add thawed contents to a single 16 x 125 mm glass screw-capped test tube of the appropriate medium. Incubate the culture vertically with the cap screwed on tightly. It is essential to establish cultures initially in small volumes. Once established, the culture can be scaled up to larger volumes. Vigorously agitate the culture and aseptically transfer 0.1 ml of culture to a fresh tube of medium weekly.

Name of Depositor FG Wallace 

Year of Origin 收藏年份 1960 

References 參考文獻(xiàn) Wallace FG, et al. Two new species of flagellates cultivated from insects of the genus Gerris. J. Protozool. 7: 390-393, 1960. 

Conchon I, et al. Trypanosomatids, other than Phytomonas spp., isolated and cultured from fruit. J. Protozool. 36: 412-414, 1989. 

Gazzinelli RT, et al. Distribution of carbohydrates recognized by the lectins Euonymus europaeus and concanavalin A in monoxenic and heteroxenic trypanosomatids. J. Protozool. 38: 320-325, 1991. PubMed: 1787421 

Camargo EP, et al. Proteolytic activities in cell extracts of trypanosomatids. J. Parasitol. 64: 1120-1121, 1978. PubMed: 739304 

Teixeira MM, Camargo EP. Monoclonal antibodies for the identification of trypanosomatids of the genus Phytomonas. J. Protozool. 36: 262-264, 1989. 

Dollahon NR, et al. Herpetomonas megaseliae, Crithidia fasciculata, and Leptomonas collosoma (Kinetoplastida, Trypanosomatidae) in feces of lizards fed culture forms of the flagellates. J. Protozool. 30: 58-62, 1983. 

Maslov DA, et al. Evolution of RNA editing in kinetoplastid protozoa: pan-editing preceded 5'-editing. Nature 368: 345-348, 1994. PubMed: 8127370 

Galinari S, Camargo EP. Trypanosomatid protozoa: survey of acetylornithinase and ornithine acetyltransferase. Exp. Parasitol. 46: 277-282, 1978. PubMed: 569594 Fish WR, et al. The cyclopropane fatty acid of trypanosomatids. Mol. Biochem. Parasitol. 3: 103-115, 1981. PubMed: 7254247 

Freymuller E, Camargo EP. Ultrastructural differences between species of trypanosomatids with and without endosymbionts. J. Protozool. 28: 175-182, 1981. PubMed: 7024533 

Camargo EP, et al. Trypanosoma spp., Leishmania spp. and Leptomonas spp.: enzymes of ornithine-arginine metabolism. Exp. Parasitol. 46: 141-144, 1978. PubMed: 569593 

Camargo EP, et al. Electrophoretic analysis of endonuclease-generated fragments of k-DNA, of esterase isoenzymes, and of surface proteins as aids for species identification of insect trypanosomatids. J. Protozool. 29: 251-258, 1982. PubMed: 6284925 type strain 

Cross References Nucleotide (GenBank) : U05812 Herpetomonas muscarum ATCC 30261 kinetoplast (MURF1) and apocytochrome B (cytB) genes, partial cds, and ATPase subunit 6 (MURF4) gene, complete pre-RNA-edited DNA sequence.