ATCC? Number:30256?
Organism: Crithidia harmosa McGhee et al.
Isolation: Euryophthalmus davisi, Athens, GA, 1969
Depositors: SH Hunter
History: ATCC<<--SH Hunter<<--R.B. McGhee
Biosafety Level:1
Shipped: frozen
Growth Conditions: ATCCmedium 355: Crithidia mediumTemperature: 25.0°C Duration: axenic Protocol: ATCCNO: 11745 SPEC: See general instructions for thawing and storage of frozen material before proceeding. Add thawed contents to a single 16 x 125 mm glass screw-capped test tube of the appropriate medium. Incubate the culture vertically with the cap screwed on tightly. It is essential to establish cultures initially in small volumes. Once established, the culture can be scaled up to larger volumes. Vigorously agitate the culture and aseptically transfer 0.1 ml of culture to a fresh tube of medium weekly.
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Comments: Ornithine-arginine metabolism [4786] Biological characteristics [4761] Riboprinting and taxonomy [23607] Nutrition of the trypanosomatids [23925] Growth on blood-agar plates [24018] Immunologic and electrophoretic characteristics [24021] Multiple distinct site-specific elements in miniexon arrays [24177] Cyclopropane fatty acid [24043]
References: 4761: McGhee RB, et al. Isolation, cloning and determination of biologic characteristics of five new species of Crithidia. J. Protozool. 16: 514-520, 1969. PubMed: 5343466 4786: Figueiredo EN, et al. Enzymes of the ornithine-arginine metabolism of trypanosomatids of the genus Crithidia. J. Protozool. 25: 546-549, 1978. 23607: Clark CG. Riboprinting: A tool for the study of genetic diversity in microorganisms. J. Eukaryot. Microbiol. 44: 277-283, 1997. PubMed: 9225441 23824: Goncanlves De Lima VM, et al. Comparison of six isoenzymes from 10 species of Crithidia. J. Protozool. 29: 397-401, 1982. 23925: Roitman I, et al. Nutrition of trypanosomatids Crithidia acanthocephali and Crithidia harmosa ribose and adenosine: Substrates for Crithidia acanthocephali. J. Protozool. 32: 490-492, 1985. 24018: Keppel AD, Janovy J J. Herpetomonas megaseliae and Crithidia harmosa: growth on blood-agar plates. J. Parasitol. 63: 879-882, 1977. PubMed: 562401 24021: Dooris PM, McGhee RB. Immunologic and electrophoretic characteristics of two species of Crithidia. J. Protozool. 23: 433-437, 1976. PubMed: 972352 24043: Fish WR, et al. The cyclopropane fatty acid of trypanosomatids. Mol. Biochem. Parasitol. 3: 103-115, 1981. PubMed: 7254247 24177: Teng SC, et al. A new non-LTR retrotransposon provides evidence for multiple distinct site-specific elements in Crithidia fasciculata miniexon arrays. Nucleic Acids Res. 23: 2929-2936, 1995. PubMed: 7659515 34222: Cho J, Eichinger D. Crithidia fasciculata induces encystation of Entamoeba invadens in a galactose-dependent manner. J. Parasitol. 84: 705-710, 1998. PubMed: 9714198