ATCC? Number:30253?
Organism: Prototheca zopfii Kruger
Designations: 48-Y
Isolation: Colgate Creek sediment, Baltimore Harbor, Chesapeake Bay, MD, 1973.
Depositors: JD Walker
Biosafety Level:1
Shipped: freeze-dried
Growth Conditions: ATCCmedium 28: Emmons' modification of Sabouraud's agarTemperature: 25.0°C Duration: axenic Protocol: ATCCNO: 16522 SPEC: This strain is distributed as a freeze-dried preparation. See the general procedures for opening a freeze-dried vial. Aseptically add 0.5 ml of ice cold medium to containing 12% sucrose to the freeze-dried inner shell vial. Once completely rehydrated, aseptically transfer the material to a 100 mm agar plate of the appropriate medium and evenly distribute the material over the surface of the agar with a spread bar. Subculture 4-6 weeks when incubated at 25C. Subculture 6-12 months when incubated at 18C To subculture, transfer a loopful of material to a fresh plate and spread evenly over the surface.
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Applications: degrades petroleum crude oil
Comments: Degrades petroleum. Petroleum-degradation [58865] Growth on acetate or n-alkanes [23771]
Classification: KINGDOM: Plantae
References: 4880: Pore RS. Prototheca taxonomy. Mycopathologia 90: 129-139, 1985. 23771: Koenig DW, Waed HB. Prototheca zopfii: Kruger strain UMK-B growth on acetate or N alkanes. Appl. Environ. Microbiol. 45: 333-336, 1983. 58865: et al., Walker JD. Petroleum-degrading achlorophyllous algae Prototheca zopfii. Nature 254: 423-424, 1975. 70536: Ueno R, et al. Optimization of heterotrophic culture conditions for n-alkane utilization and phylogenetic position based on the 18S rDNA sequence of a thermotolerant Prototheca zopfii strain. J. Biosci. Bioeng. 94: 160-165, 2002.