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平臺編號：bio-48577
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國際編號：ATCC 30241
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拉丁屬名： Trichomonas vaginalis
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規(guī)格：frozen
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用途：ATCC原裝進口
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服務(wù)費用：
加載中…… - 訂購
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注意事項：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準）
JH 37A #4
Trichomonas vaginalis Donne 拉丁名
(ATCC? 30241?) 統(tǒng)一編號
Strain Designations 菌種別名 JH 37A #4
Application 用途 Sexually Transmitted Disease Research
Biosafety Level 生物安全等級 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Isolation 分離源 Endocervical swab, Johns Hopkins Hosp., Baltimore, MD, 1964
Product Format 提供形式 frozen
Storage Conditions 保存方法
Frozen Cultures 冷凍物 : -70°C for 1 week; liquid N2 vapor for long term storage
Freeze-dried Cultures 凍干物 : 2-8°C
Live Cultures 活菌 : See Protocols section for handling information
Type Strain 模式菌株 no
Medium 培養(yǎng)基 ATCC? Medium 2154: LYI Entamoeba medium
Growth Conditions 生長條件
Temperature 培養(yǎng)溫度 : 35°C
Atmosphere 需氧情況 : Anaerobic
Culture System: Axenic; pH 6.0
Cryopreservation Harvest and Preservation Harvest cells from a culture that is at or near peak density by centrifugation at 800 x g for 5 min.
The cells grown in a medium containing agar are concentrated by centrifugation, a solid pellet does not form. The soft pellet is resuspended to desired cell concentration with agar-free supernatant.
Adjust the concentration of cells to 2 x 106 to 2 x 107/mL in fresh medium. While cells are centrifuging prepare a 10% (v/v) solution of sterile DMSO in fresh medium.
Add 1.0 mL of DMSO to an ice cold 20 x 150 mm screw-capped test tube. Place the tube on ice and allow the DMSO to solidify (~5 min) and then add 9.0 mL of ice cold medium.
Invert several times to dissolve the DMSO. Allow to warm to room temperature. Mix the cell preparation and the DMSO in equal portions. Thus, the final concentration will be 106 to 107 cells/mL and 5% (v/v) DMSO.
The time from the mixing of the cell preparation and DMSO stock solution before the freezing process is begun should no less than 15 min and no longer than 30 min.
Dispense in 0.5 mL aliquots into 1.0 mL to 2.0 mL sterile plastic screw-capped cryules (special plastic vials for cryopreservation). Place the vials in a controlled rate freezing unit.
From room temperature cool at -1°C/min to -40°C. If the freezing unit can compensate for the heat of fusion, maintain rate at -1°C/min through the heat of fusion.
At -40°C plunge into liquid nitrogen. Alternatively, place the vials in a Nalgene 1°C freezing apparatus. Place the apparatus at -80°C for 1.5 to 2 hours and then plunge ampules into liquid nitrogen. (The cooling rate in this apparatus is approximately -1°C/min.)
The frozen preparations should be stored in either the vapor or liquid phase of a nitrogen refrigerator. Frozen preparations stored below -130°C are stabile indefinitely. Those stored at temperatures above -130°C are progressively less stabile as the storage temperature is elevated. Vials should not be stored above -55°C.
To establish a culture from the frozen state place an ampule in a water bath set at 35°C. Immerse the vial just to a level just above the surface of the frozen material. Do not agitate the vial.
Immediately after thawing, do not leave in the water bath, aseptically remove the contents of the ampule and inoculate a 16 x 125 mm screw-capped test tube containing either 9 mL of ATCC medium 361 (completed with serum) or 13 mL ATCC Medium 2154 adjusted to pH 6.0.
Incubate the culture at 35°C with the cap screwed on tightly (tube should be vertical for medium 361 or on a 15° horizontal slant for medium 2154).
Name of Depositor 寄存人 BM Honigberg
Year of Origin 1964
References 參考文獻 Felleisen RS, et al. Detection of Tritrichomonas foetus by PCR and DNA enzyme immunoassay based on rRNA gene unit sequences. J. Clin. Microbiol. 36: 513-519, 1998. PubMed: 9466768
Felleisen RS. Comparative sequence analysis of 5.8S rRNA genes and internal transcribed spacer (ITS) regions of trichomonadid protozoa. Parasitology 115: 111-119, 1997. PubMed: 10190167
