ATCC? Number:30251?
Organism: Crithidia acanthocephali Hanson and McGhee
Isolation: Acanthocephala femorata, Athens, GA, 1959
Depositors: SH Hunter
Biosafety Level:1
Shipped: frozen
Growth Conditions: ATCCmedium 355: Crithidia mediumTemperature: 25.0°C Duration: axenic Protocol: ATCCNO: 11745 SPEC: See general instructions for thawing and storage of frozen material before proceeding. Add thawed contents to a single 16 x 125 mm glass screw-capped test tube of the appropriate medium. Incubate the culture vertically with the cap screwed on tightly. It is essential to establish cultures initially in small volumes. Once established, the culture can be scaled up to larger volumes. Vigorously agitate the culture and aseptically transfer 0.1 ml of culture to a fresh tube of medium weekly.
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Comments: Ornithine-arginine metabolism [4786] species description [4694] Trypanosomatids from fruit [4818] Riboprinting and taxonomy [23607] Monoclonal antibodies for identification [23826] Nutrition of the trypanosomatids [23925] Proteolytic activities [23776] Acetylornithinase and ornithine acetyltransferase [24012] Cyclopropane fatty acid [24043] Ultrastructural differences between species with and without endosymbionts [24077] isoleucine requirement and threonine deaminase [24118] Multiple distinct site-specific elements in miniexon arrays [24177] endonuclease-generated fragments of K-DNA, esterase isoenzymes, surface proteins for species identification [24216]
References: 4694: Hanson and McGhee. The biology and morphology of Crithidia acanthocephali n. sp., Leptomonas leptoglossi n. sp., and Blastocrithidia euschisti n. sp.. J. Protozool. 8: 200-204, 1961. 4786: Figueiredo EN, et al. Enzymes of the ornithine-arginine metabolism of trypanosomatids of the genus Crithidia. J. Protozool. 25: 546-549, 1978. 4818: Conchon I, et al. Trypanosomatids, other than Phytomonas spp., isolated and cultured from fruit. J. Protozool. 36: 412-414, 1989. 23607: Clark CG. Riboprinting: A tool for the study of genetic diversity in microorganisms. J. Eukaryot. Microbiol. 44: 277-283, 1997. PubMed: 9225441 23776: Camargo EP, et al. Proteolytic activities in cell extracts of trypanosomatids. J. Parasitol. 64: 1120-1121, 1978. PubMed: 739304 23824: Goncanlves De Lima VM, et al. Comparison of six isoenzymes from 10 species of Crithidia. J. Protozool. 29: 397-401, 1982. 23826: Teixeira MM, Camargo EP. Monoclonal antibodies for the identification of trypanosomatids of the genus Phytomonas. J. Protozool. 36: 262-264, 1989. 23925: Roitman I, et al. Nutrition of trypanosomatids Crithidia acanthocephali and Crithidia harmosa ribose and adenosine: Substrates for Crithidia acanthocephali. J. Protozool. 32: 490-492, 1985. 24012: Galinari S, Camargo EP. Trypanosomatid protozoa: survey of acetylornithinase and ornithine acetyltransferase. Exp. Parasitol. 46: 277-282, 1978. PubMed: 569594 24043: Fish WR, et al. The cyclopropane fatty acid of trypanosomatids. Mol. Biochem. Parasitol. 3: 103-115, 1981. PubMed: 7254247 24077: Freymuller E, Camargo EP. Ultrastructural differences between species of trypanosomatids with and without endosymbionts. J. Protozool. 28: 175-182, 1981. PubMed: 7024533 24118: Alfieri SC, Camargo EP. Trypanosomatidae: isoleucine requirement and threonine deaminase in species with and without endosymbionts. Exp. Parasitol. 53: 371-380, 1982. PubMed: 6806116 24177: Teng SC, et al. A new non-LTR retrotransposon provides evidence for multiple distinct site-specific elements in Crithidia fasciculata miniexon arrays. Nucleic Acids Res. 23: 2929-2936, 1995. PubMed: 7659515 24216: Camargo EP, et al. Electrophoretic analysis of endonuclease-generated fragments of k-DNA, of esterase isoenzymes, and of surface proteins as aids for species identification of insect trypanosomatids. J. Protozool. 29: 251-258, 1982. PubMed: 6284925 34222: Cho J, Eichinger D. Crithidia fasciculata induces encystation of Entamoeba invadens in a galactose-dependent manner. J. Parasitol. 84: 705-710, 1998. PubMed: 9714198