ATCC® Number:30208™
Organism: Trypanosoma cruzi Chagas
Designations: Tulahuen
Isolation: Tulahuen, Chile, 1949
Depositors: TI Mercado
History: ATCC<<--TI Mercado<<--A. Szarfman <<--- . . . <<--- T. Pizzi
Biosafety Level:2
Shipped: frozen
Growth Conditions: Duration: in vivo, laboratory mouse Protocol: ATCCNO: 30208 SPEC: This strain is distributed as a frozen stabilate. See general instructions for thawing and storage of frozen material before proceeding. Upon arrival remove the frozen ampoule from the dry ice and transfer directly to a 35C water bath. When completely thawed, aseptically remove the material with a syringe and inject 0.2 ml aliquots of the thawed contents of the ampule intraperitoneally into an adult rats known to be free of blood parasites. Draw a drop of blood from the tail of an infected rat. Add 1.0 ml of mammalian saline (0.8% saline buffered to pH 7.0) to the blood and examine a small amount microscopically. If one or more parasites are seen, proceed as follows: collect parasites into a syringe containing a buffered anticoagulant by either cardiac puncture or from the tail vein of the infected animal. Inoculate 0.2 ml of the diluted blood intraperitoneally into an adult rat known to be free of other blood parasites. Repeat the above procedures approximately every 4 days. For T. cruzi, parasites for inoculation should be collected at peak parasitemia (usually 6-9 days) and inoculated into 4- to 6-week-old mice.
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments: Lysis caused by antitrypanosoma factor ATF-II [4567]
Classification: KINGDOM: Protozoa
References: 4567: Mercado TI, Colon-Whitt A. Lysis of trypanosoma cruzi. US Patent 4,474,772 dated Oct 2 1984 5057: Mercado TI. Calcium in the liver of mice infected with Trypanosoma cruzi. J. Parasitol. 58: 1137-1142, 1972. PubMed: 4345111
