ATCC® Number:30234™
Organism: Acanthamoeba castellanii (Douglas) Page
Isolation: derived from ATCC30011
Depositors: W Balamuth
Biosafety Level:1
Shipped: frozen
Growth Conditions: ATCCmedium 712: PYGTemperature: 25.0°C Growth Conditions: Axenic Protocol: This strain is distributed as a frozen preparation. See the general procedures for thawing a frozen vial. Once vial is thawed, aseptically transfer the entire contents to the center of an agar plate of ATCCmedium 711 and spread it evenly over the surface of the plate with a spread bar. Incubate the plate at 25C. Trophozoites (amoebae) should be evident within 2-3 days.
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Comments: infection by Chlamydia pneumoniae [38798] enhancement of virulence of Mycobacterium avium [29894] Intracellular growth of Legionella pneumophila [4709] Survival of bacterial pathogens within protozoa [4927] phylogeny [24028] Growth of Legionella pneumophila [24067]
Classification: KINGDOM: Protozoa
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