ATCC® Number:30224™
Organism: Naegleria gruberi Schardinger
Designations: NEG-M
Isolation: derived in 1969 from strain NEG (See ATCC30223)
Depositors: C Fulton
Biosafety Level:1
Shipped: frozen
Growth Conditions: ATCCmedium 803: M7 mediumTemperature: 25.0°C Duration: axenic
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Comments: Chemically defined medium [5028] Circular ribosomal RNA genes [4814] rRNA Genes on 14-Kilobase- Pair Plasmid [4888] Cell differentiation [23734] Freeze preservation of pathogenic and nonpathogenic species [23993]
Cross References: Nucleotide (GenBank) : M18732 N.gruberi 18S subunit ribosomal RNA gene.
References: 4814: Clark CG, Cross GA. Circular ribosomal RNA genes are a general feature of schizopyrenid amoebae. J. Protozool. 35: 326-329, 1988. PubMed: 2840492 4888: Clark CG, Cross GA. rRNA genes of Naegleria gruberi are carried exclusively on a 14- kilobase-pair plasmid. Mol. Cell. Biol. 7: 3027-3031, 1987. PubMed: 2823115 5028: Fulton C, et al. Chemically defined media for cultivation of Naegleria gruberi. Proc. Natl. Acad. Sci. USA 81: 2406-2410, 1984. 5136: Fulton C. Methods in cell physiology. vol. 4New York: Academic Press; 1970. 23734: Fulton C. Cell differentiation in Naegleria gruberi. Annu. Rev. Microbiol. 31: 597-629, 1977. PubMed: 334046 23993: Simione FP Jr., Daggett PM. Freeze preservation of pathogenic and nonpathogenic Naegleria species. J. Parasitol. 62: 49, 1976. PubMed: 1255382 61465: De Jonckheere JF. A century of research on the amoeboflagellate genus Naegleria. Acta Protozool. 41: 309-342, 2002. 70544: Simpson AGB, et al. The evolutionary history of kinetoplastids and their kinetoplasts. Mol. Biol. Evol. 19: 2071-2083, 2002. PubMed: 12446799