ATCC? Number:30221?
Organism: Babesia microti (Franca) Reichenow
Designations: Gray
Isolation: human, Nantucket Island, MA, 1970
Depositors: JP Kreier
History: ATCC<<--JP Kreier<<--A. Holbrook <<--- . . . <<--- K.A. Western
Biosafety Level:2
Shipped: frozen
Growth Conditions: Duration: in vivo, Golden Syrian hamster Protocol: ATCCNO: 30221 SPEC: This strain is distributed as a frozen stabilate. See general instructions for thawing and storage of frozen material before proceeding. Upon arrival remove the frozen ampoule from the dry ice and transfer directly to a 35C water bath. When completely thawed, aseptically remove the material with a syringe and inject the entire contents intraperitoneally into a young hamster. Remove parasitized blood via cardiac puncture and inject 2.5 X 10(6) or greater parasitized erythrocytes from donor into the appropriate host at weekly intervals.
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Comments: Infection with piroplasms isolated from man [5054]
Cross References: Nucleotide (GenBank) : U53448 Babesia microti heat shock protein 70 (hsp70) gene, complete cds.
Classification: KINGDOM: Protozoa
References: 5054: Van Peenen PF, Healy GR. Infection of Microtus ochrogaster with piroplasms isolated from man. J. Parasitol. 56: 1029-1031, 1970. PubMed: 5504524