ATCC? Number:30223?
Organism: Naegleria gruberi Schardinger
Designations: NEG
Isolation: derived in 1967 from W. Balamuth strain EG (ATCC30133)
Depositors: C Fulton
Biosafety Level:1
Shipped: frozen
Growth Conditions: ATCCmedium 997: Fresh water ameba mediumTemperature: 25.0°C Duration: grown with Escherichia coli ATCC11775
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Comments: Zymodeme Gr-9 [23932] Maximum temperature tolerance 37C [23932] Maximum temperature tolerance 39C [38900] Cell differentiation [23734] Genetic cluster L [Int. J. Parasitol. 19: 823-834, 1989] = Naegleria gruberi cluster 1 [J. Eukaryot. Microbiol. 40: 179-187, 1993]. Species-specific antibodies to actin [24005]
References: 5136: Fulton C. Methods in cell physiology. vol. 4New York: Academic Press; 1970. 23734: Fulton C. Cell differentiation in Naegleria gruberi. Annu. Rev. Microbiol. 31: 597-629, 1977. PubMed: 334046 23861: De Jonckheere JF. A Group I intron in the SSUrDNA of some Naegleria spp. demonstrated by polymerase chain reaction amplification. J. Eukaryot. Microbiol. 40: 179-187, 1993. PubMed: 8461891 23932: Adams M, et al. A genetic approach to species criteria in the amoeba genus Naegleria using allozyme electrophoresis. Int. J. Parasitol. 19: 823-834, 1989. PubMed: 2635158 24005: Fulton C, et al. Naegleria actin elicits species-specific antibodies. J. Protozool. 33: 322-327, 1986. PubMed: 2427713 38900: Robinson BS, et al. Discontinuous genetic variation among mesophilic Naegleria isolates: Further evidence that N. gruberi is not a single species. J. Protozool. 39: 702-712, 1992. PubMed: 1453360 61465: De Jonckheere JF. A century of research on the amoeboflagellate genus Naegleria. Acta Protozool. 41: 309-342, 2002.